PXD009875 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Optimizing chromatographic conditions for orbitrap-based MS platforms |
Description | We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa cell protein extract, we carried out 16 different chromatography conditions on four different instrumentation platforms with three replicates of each condition, for a total of 48 proteomics analyses. We first compared selected chromatography conditions of the QE+ and OFL by varying column lengths, inner diameters, and C18-RP particle sizes. We next selected one chromatography condition on each system and varied the effective RP gradients lengths |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-04 |
AnnouncementXML | Submission_2019-03-04_02:30:58.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD009875 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Laurence Florens |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos; LTQ Orbitrap Velos; Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-24 01:05:30 | ID requested | |
⏵ 1 | 2019-03-04 02:30:59 | announced | |
2 | 2019-03-26 09:47:36 | announced | Updated publication reference for PubMed record(s): 30884231. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Technical |
submitter keyword: Orbitrap, Q Exactive, Fusion Lumos, Quantitative Proteomics, Human, Chromatography, Pearson Product-Moment Correlation Coefficient, Reproducibility, Multidimensional Protein Identification Technology, distributed Normalized Spectral Abundance Factor, Data-Dependent Acquisition |
Contact List
Laurence Florens |
contact affiliation | Stowers Institute for Medical Research |
contact email | laf@stowers.org |
lab head | |
Laurence Florens |
contact affiliation | Proteomics |
contact email | laf@stowers.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009875
- Label: PRIDE project
- Name: Optimizing chromatographic conditions for orbitrap-based MS platforms