PXD009785

PXD009785 is an original dataset announced via ProteomeXchange.

Title	LED lamps induce a differential regulation of proteins and enhance somatic embryo maturation in Carica papaya
Description	The use of light-emitting diode (LED) lamps could be an alternative method to improve somatic embryogenesis, yielding more efficient somatic embryo development and maturation than the use of conventional fluorescent lamps. The aim of this work was to study the influence of light quality on the somatic embryo development and differential abundance of proteins during the maturation of papaya (Carica papaya) cv. ‘Golden’ embryogenic cultures, using LED lamps with different wavelengths. Of all the LED treatments, the white plus medium blue (WmB - 450/530 nm) light resulted in the best somatic embryo production after 28 days of maturation. The WmB and fluorescent treatments generated 82.4 and 47.6 cotyledonary stage somatic embryos, respectively. By a comparative shotgun proteomics analysis between WmB LED and fluorescent lamps treatments, a total of 28 up-regulated and 7 down-regulated proteins were identified. Among the proteins up-regulated in the cultures treated with WmB LED light compared with fluorescent light were the indole-3-acetic acid-amido synthetase (GH3), pyrophosphate-energized vacuolar membrane proton pump (H+-PPase), and actin-depolymerizing factor 2 (ADF2) proteins, which are involved in the regulation of auxin levels by auxin conjugation and transport. Additionally, proteins related to energetic supply, protein metabolism, cell wall remodeling, internal trafficking, and cell division were up-regulated, showing a significantly higher abundance in the embryogenic cultures incubated under WmB LED light than those incubated under fluorescent light.
HostingRepository	PRIDE
AnnounceDate	2019-09-11
AnnouncementXML	Submission_2019-09-11_10:12:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanildo Silveira
SpeciesList	scientific name: Carica papaya; NCBI TaxID: 3649;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-15 03:52:17	ID requested
⏵ 1	2019-09-11 10:12:13	announced

Almeida FA, Vale EM, Reis RS, Santa-Catarina C, Silveira V, LED lamps enhance somatic embryo maturation in association with the differential accumulation of proteins in the Carica papaya L. 'Golden' embryogenic callus. Plant Physiol Biochem, 143():109-118(2019) [pubmed]

curator keyword: Biological

submitter keyword: Carica papaya L., Clonal propagation, Comparative proteomics LED, Label-free proteomics, Light-emitting diode, Somatic embryogenesis

Vanildo Silveira
contact affiliation	Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, Campos dos Goytacazes, RJ, Brazil. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil.
contact email	vanildo@uenf.br
lab head
Vanildo Silveira
contact affiliation	UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE
contact email	vanildo@uenf.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009785
     1. Label: PRIDE project
     2. Name: LED lamps induce a differential regulation of proteins and enhance somatic embryo maturation in Carica papaya