PXD009721 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In-depth quantitative proteomic analysis of trophozoites and pseudocysts of Trichomonas vaginalis r |
Description | Trichomonas vaginalis is a sexually transmitted anaerobic parasite that infects humans causing trichomoniasis, a common and ubiquitous sexually transmitted disease. The life cycle of this parasite presents a trophozoite form without a cystic stage. However, the presence of spherical forms with internalized flagella, non-proliferative, non-motile, viable and reversible, denominated pseudocysts, have been commonly observed in this parasite. To understand the mechanisms involved in the formation of pseudocysts, here we performed a mass spectrometry-based high-throughput quantitative proteomics study using a label-free approach and functional assays by biochemical and flow cytometric methods. We observed that morphological transformation of trophozoite to pseudocysts is coupled to (i) a metabolic shift toward a less glycolytic phenotype, (ii) alterations in the abundance of hydrogenosomal iron-sulfur cluster (ISC) assembly machinery; (iii) increased abundance of regulatory particles of the ubiquitin–proteasome system; (iv) significant alterations in proteins involved in adhesion and cytoskeleton reorganization; (v) arrest in G2/M phase associated to alterations in the abundance of regulatory proteins of the cell cycle. Such data supports that pseudocysts suffer important physiological and structural alterations for survive under unfavorable environmental conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2022-03-01 |
AnnouncementXML | Submission_2022-03-01_09:41:55.292.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jacek Wisniewski |
SpeciesList | scientific name: Trichomonas vaginalis; NCBI TaxID: 5722; |
ModificationList | monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-08 23:52:06 | ID requested | |
⏵ 1 | 2022-03-01 09:41:56 | announced | |
Publication List
Dias-Lopes G, Wi, ś, niewski JR, de Souza NP, Vidal VE, Padr, ó, n G, Britto C, Cuervo P, De Jesus JB, In-Depth Quantitative Proteomic Analysis of Trophozoites and Pseudocysts of Trichomonas vaginalis. J Proteome Res, 17(11):3704-3718(2018) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Trichomonas vaginalis, parasite, total protein approach, proteomic ruler, FASP |
Contact List
Jacek R Wisniewski |
contact affiliation | Max Planck Institute of Biochemistry Martinsried, Germany |
contact email | jwisniew@biochem.mpg.de |
lab head | |
Jacek Wisniewski |
contact affiliation | Proteomics |
contact email | jwisniew@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009721
- Label: PRIDE project
- Name: In-depth quantitative proteomic analysis of trophozoites and pseudocysts of Trichomonas vaginalis r