PXD009721

PXD009721 is an original dataset announced via ProteomeXchange.

Title	In-depth quantitative proteomic analysis of trophozoites and pseudocysts of Trichomonas vaginalis r
Description	Trichomonas vaginalis is a sexually transmitted anaerobic parasite that infects humans causing trichomoniasis, a common and ubiquitous sexually transmitted disease. The life cycle of this parasite presents a trophozoite form without a cystic stage. However, the presence of spherical forms with internalized flagella, non-proliferative, non-motile, viable and reversible, denominated pseudocysts, have been commonly observed in this parasite. To understand the mechanisms involved in the formation of pseudocysts, here we performed a mass spectrometry-based high-throughput quantitative proteomics study using a label-free approach and functional assays by biochemical and flow cytometric methods. We observed that morphological transformation of trophozoite to pseudocysts is coupled to (i) a metabolic shift toward a less glycolytic phenotype, (ii) alterations in the abundance of hydrogenosomal iron-sulfur cluster (ISC) assembly machinery; (iii) increased abundance of regulatory particles of the ubiquitin–proteasome system; (iv) significant alterations in proteins involved in adhesion and cytoskeleton reorganization; (v) arrest in G2/M phase associated to alterations in the abundance of regulatory proteins of the cell cycle. Such data supports that pseudocysts suffer important physiological and structural alterations for survive under unfavorable environmental conditions.
HostingRepository	PRIDE
AnnounceDate	2022-03-01
AnnouncementXML	Submission_2022-03-01_09:41:55.292.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jacek Wisniewski
SpeciesList	scientific name: Trichomonas vaginalis; NCBI TaxID: 5722;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-08 23:52:06	ID requested
⏵ 1	2022-03-01 09:41:56	announced

Dias-Lopes G, Wi, ś, niewski JR, de Souza NP, Vidal VE, Padr, ó, n G, Britto C, Cuervo P, De Jesus JB, In-Depth Quantitative Proteomic Analysis of Trophozoites and Pseudocysts of Trichomonas vaginalis. J Proteome Res, 17(11):3704-3718(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Trichomonas vaginalis, parasite, total protein approach, proteomic ruler, FASP

Jacek R Wisniewski
contact affiliation	Max Planck Institute of Biochemistry Martinsried, Germany
contact email	jwisniew@biochem.mpg.de
lab head
Jacek Wisniewski
contact affiliation	Proteomics
contact email	jwisniew@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009721
     1. Label: PRIDE project
     2. Name: In-depth quantitative proteomic analysis of trophozoites and pseudocysts of Trichomonas vaginalis r