PXD009680

PXD009680 is an original dataset announced via ProteomeXchange.

Title	iTRAQ Protemics of mdx vs. mdx/Actg1-TG mouse skeletal muscle
Description	Duchenne muscular dystrophy (DMD) is an X-linked recessive disease caused by deleterious mutations in the DMD gene, rendering non-functional forms or complete absence of the protein dystrophin. Eccentric contraction-induced force loss is the most robust and reproducible phenotype of dystrophin-deficient skeletal muscle, yet the molecular mechanisms underlying force loss remain obscure. To this end, we utilized the mdx mouse model of DMD, which displays extreme sensitivity to eccentric contractions. An existing mouse line from our lab that overexpresses cytoplasmic gamma-actin specifically in skeletal muscle (mdx/Actg1-TG) was shown to significantly protect mdx muscle against contraction-induced force loss. To understand the mechanism behind this protection, we performed iTRAQ proteomics on mdx/Actg1-TG tibialis anterior (TA) muscle versus non-transgenic littermate controls to identify differentially-expressed proteins that may afford protection upon gamma-actin overexpression.
HostingRepository	PRIDE
AnnounceDate	2020-11-10
AnnouncementXML	Submission_2020-11-10_06:10:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Ervasti
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-04 07:37:06	ID requested
1	2018-10-22 04:33:55	announced
⏵ 2	2020-11-10 06:10:27	announced	2020-11-10: Updated publication reference for PubMed record(s): 30504831.

Olthoff JT, Lindsay A, Abo-Zahrah R, Baltgalvis KA, Patrinostro X, Belanto JJ, Yu DY, Perrin BJ, Garry DJ, Rodney GG, Lowe DA, Ervasti JM, Loss of peroxiredoxin-2 exacerbates eccentric contraction-induced force loss in dystrophin-deficient muscle. Nat Commun, 9(1):5104(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Skeletal muscle, mdx, iTRAQ

James M. Ervasti
contact affiliation	Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN, USA
contact email	jervasti@umn.edu
lab head
James Ervasti
contact affiliation	University of Minnesota
contact email	ervastilab@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD009680

PRIDE project URI

• PRIDE
  1. PXD009680
     1. Label: PRIDE project
     2. Name: iTRAQ Protemics of mdx vs. mdx/Actg1-TG mouse skeletal muscle