PXD009659

PXD009659 is an original dataset announced via ProteomeXchange.

Title	A survival mechanism controlled by cadherins and Dlg1 polarity complex inhibits Fas cell death receptor signaling
Description	A fine regulation of epithelia cell death is required to maintain tissue integrity and homeostasis. At a cellular level, this life and death decision is controlled by environmental stimuli including death receptors activation. Here, we show that establishment of cell polarity and AJ formation control the pro-apoptotic signaling emanating from the death receptor Fas. We demonstrate that in colon epithelia Fas concentrates at cell-cell junctions together with the E-cadherin, which protects cells from FasL-induced cell death. The Fas-cadherin association requires the C terminal PDZ binding site of Fas and, using a proteomic approach, we showed that this domain allows the association with the polarity molecule Dlg1. We proved that the interaction of Fas with this scaffold molecule participate to this cell death protection. Therefore inhibition of FasL-induced cell death by Fas-cadherin-Dlg1 complex is a double-edged sword mechanism that helps to maintain epithelial homeostasis by (i) protecting normal polarized epithelia from apoptosis (ii) promoting the elimination of compromised non-polarized cells.
HostingRepository	PRIDE
AnnounceDate	2021-05-28
AnnouncementXML	Submission_2021-05-27_21:56:44.650.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AUDEBERT Stephane
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-03 01:42:50	ID requested
1	2018-09-20 05:09:28	announced
⏵ 2	2021-05-27 21:56:45	announced	2021-05-28: Updated publication reference for PubMed record(s): 30242034.

Gagnoux-Palacios L, Awina H, Audebert S, Rossin A, Mondin M, Borgese F, Planas-Botey C, Mettouchi A, Borg JP, Hueber AO, Cell polarity and adherens junction formation inhibit epithelial Fas cell death receptor signaling. J Cell Biol, 217(11):3839-3852(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, LC-MSMS, PDZ, cell Polarity, epithelia, cell death, death receptor

Audebert Stephane
contact affiliation	Centre de Recherche en Cancérologie de Marseille, CRCM Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France
contact email	stephane.audebert@inserm.fr
lab head
AUDEBERT Stephane
contact affiliation	Pharmacology department
contact email	stephane.audebert@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009659

PRIDE project URI

• PRIDE
  1. PXD009659
     1. Label: PRIDE project
     2. Name: A survival mechanism controlled by cadherins and Dlg1 polarity complex inhibits Fas cell death receptor signaling