PXD009540 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Rapamycin regulate cholesterol metabolism and ribosomal proteins in Alzheimer’s disease |
Description | In order to disclose the mechanisms, a high-throughput quantitative proteomics analysis was established to investigate the proteome profiles changes of hippocampus and temporal lobe of WT mice, APP/PS1 mice and rapamycin treated APP/PS1 mice.The extraction of proteins of hippocampus and temporal tissue employed liquid nitrogen grounding method, which was described as previous works of our laboratory and other laboratories [20, 21]. After fully and immediately grinding under the protection of liquid nitrogen, the products was dissolved by lysis buffer (8 M urea and 1 cocktail in PBS, pH = 8.0) prepared in ice ahead of schedule and then the solutions were transferred to a 1.5-mL tube in ice. Cellular debris was removed by centrifugation (12,000 rpm, 15 min, 4 °C) and the liquid supernatant was transferred into a fresh 1.5-mL tube and immediately stored at -80 °C. The protein concentration of the supernatant was detected by Nanodrop 2000 (Thermo Scientific, NJ, USA) following the manufacture instructions. |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-28 |
AnnouncementXML | Submission_2019-02-28_09:47:38.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wei Ge |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-04-19 02:28:28 | ID requested | |
⏵ 1 | 2019-02-28 09:47:39 | announced | |
Publication List
Wang X, Xia W, Li K, Zhang Y, Ge W, Ma C, Rapamycin regulates cholesterol biosynthesis and cytoplasmic ribosomal proteins in hippocampus and temporal lobe of APP/PS1 mouse. J Neurol Sci, 399():125-139(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Alzheimer’s disease, Rapamycin, Protomics, Hippocampus, Temporal lobe |
Contact List
Wei Ge |
contact affiliation | National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Dongcheng District, Beijing 100005, China. |
contact email | wei.ge@chem.ox.ac.uk |
lab head | |
Wei Ge |
contact affiliation | National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences |
contact email | wei.ge@chem.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009540
- Label: PRIDE project
- Name: Rapamycin regulate cholesterol metabolism and ribosomal proteins in Alzheimer’s disease