PXD009498 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SILAC APMS analysis of CCDC103 interactome |
Description | To get insights into the function of CCD103, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-CCDC103 and control cells with isotopically labelled amino-acids (SILAC), whole cell extracts were immuno-precipitated (IP) with anti-GFP antibodies and immunoprecipitates were subjected to quantitative mass-spectrometry analysis. SILAC labels: K0R0 control Hela H9 vs K4R6 x-FLAG- GFP-CCDC103 |
HostingRepository | PRIDE |
AnnounceDate | 2018-06-08 |
AnnouncementXML | Submission_2018-06-08_03:06:47.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Franck Vandermoere |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | isotope labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-04-13 05:30:52 | ID requested | |
⏵ 1 | 2018-06-08 03:06:48 | announced | |
Publication List
Roitbak AI, Eristavi N, EEG and behavior reactions upon stimulation of nonspecific thalamic nuclei in unanesthetized cats. Acta Biol Exp (Warsz), 26(4):463-82(1966) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Human, Hela cells, SILAC, APMS, LTQ-Orbitrap |
Contact List
Edouard Bertrand |
contact affiliation | IGMM, CNRS, Université de Montpellier, Montpellier, France |
contact email | edouard.bertrand@igmm.cnrs.fr |
lab head | |
Franck Vandermoere |
contact affiliation | CNRS |
contact email | franck.vandermoere@igf.cnrs.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009498
- Label: PRIDE project
- Name: SILAC APMS analysis of CCDC103 interactome