PXD009498

PXD009498 is an original dataset announced via ProteomeXchange.

Title	SILAC APMS analysis of CCDC103 interactome
Description	To get insights into the function of CCD103, we characterized its partners by performing a proteomic analysis in human cells. We fused this domain to GFP and stably expressed it in HeLa cells using site-specific integration with the Flp-In system. Following differential labeling of GFP-CCDC103 and control cells with isotopically labelled amino-acids (SILAC), whole cell extracts were immuno-precipitated (IP) with anti-GFP antibodies and immunoprecipitates were subjected to quantitative mass-spectrometry analysis. SILAC labels: K0R0 control Hela H9 vs K4R6 x-FLAG- GFP-CCDC103
HostingRepository	PRIDE
AnnounceDate	2018-06-08
AnnouncementXML	Submission_2018-06-08_03:06:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Franck Vandermoere
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	isotope labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-13 05:30:52	ID requested
⏵ 1	2018-06-08 03:06:48	announced

Roitbak AI, Eristavi N, EEG and behavior reactions upon stimulation of nonspecific thalamic nuclei in unanesthetized cats. Acta Biol Exp (Warsz), 26(4):463-82(1966) [pubmed]

curator keyword: Biological

submitter keyword: Human, Hela cells, SILAC, APMS, LTQ-Orbitrap

Edouard Bertrand
contact affiliation	IGMM, CNRS, Université de Montpellier, Montpellier, France
contact email	edouard.bertrand@igmm.cnrs.fr
lab head
Franck Vandermoere
contact affiliation	CNRS
contact email	franck.vandermoere@igf.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009498

PRIDE project URI

• PRIDE
  1. PXD009498
     1. Label: PRIDE project
     2. Name: SILAC APMS analysis of CCDC103 interactome