PXD009448

PXD009448 is an original dataset announced via ProteomeXchange.

Title	Thylakoid membrane proteome remodelling in response to variation in plant growth irradiance
Description	Plant thylakoid membranes contain hundreds of proteins closely interplaying to cope with ever-changing environmental conditions. We investigated how P. sativum (pea) grown at different irradiances optimizes light-use efficiency through the differential accumulation of thylakoid proteins. Thylakoid membranes from plants grown under limiting (LL), normal (NL) and high (HL) light intensity were characterized by combining chlorophyll fluorescence measurements with quantitative proteomic analysis. Protein sequences retrieved from available transcriptomic data considerably improved the protein profiling. We found that increasing growth irradiance affects the electron transport kinetics but not Photosystem (PS) I and II relative abundance. Two acclimation strategies were evident comparing plants acclimated to LL with higher irradiances: 1) in NL, plants turn on photoprotective responses mostly regulating the PSII light-harvesting capacity either accumulating Lhcb4.3 or favouring the xanthophyll cycle; 2) in HL, plants reduce the LHCII pool and enhance the PSII repair cycle. At increasing growth irradiance, plants increase the accumulation of ATP synthase and boost the electron transport to finely tune the ΔpH across the membrane and adjust the thylakoid architecture to optimize protein trafficking. Our results provide a quantitative snapshot on how plants coordinate light-harvesting, electron transport and protein synthesis adjusting the thylakoid membrane proteome in a light-dependent manner
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:23.779.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pascal Albanese
SpeciesList	scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-10 01:39:26	ID requested
1	2018-10-02 06:06:46	announced
2	2018-10-05 05:51:38	announced	Updated publication reference for DOI(s): 10.1111/tpj.14068.
⏵ 3	2023-11-14 08:49:24	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: thylakoid membrane, SWATH analysis, photosynthesis, quantitative proteomics,light acclimation

Cristina Pagliano
contact affiliation	Applied Science and Technology Department–BioSolar Lab, Politecnico di Torino,Italy
contact email	cristina.pagliano@polito.it
lab head
Pascal Albanese
contact affiliation	Politecnico di Torino
contact email	pascal.albanese@polito.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009448
     1. Label: PRIDE project
     2. Name: Thylakoid membrane proteome remodelling in response to variation in plant growth irradiance