PXD009379-1

PXD009379 is an original dataset announced via ProteomeXchange.

Title	Metabolic reprogramming of Vibrio cholerae impaired in respiratory NADH oxidation is accompanied with increased copper sensitivity
Description	The electrogenic, sodium ion translocating NADH:quinone oxidoreductase (NQR) from Vibrio cholerae is frequent in pathogenic bacteria and a potential target for antibiotics. NQR couples the oxidation of NADH to the formation of a sodium motive force (SMF) and therefore drives important processes such as flagellar rotation, substrate uptake, and energy-dissipating cation-proton antiport. We performed a quantitative proteome analysis of V. cholerae O395N1 in comparison to its variant lacking the NQR using minimal medium with glucose as carbon source. We found 84 proteins (≥ regulation factor 2) to be changed in abundance. The loss of NQR resulted in a decrease in abundance of enzymes of the oxidative branch of the TCA cycle and an increase in abundance of virulence factors AcfC and TcpA. Most unexpected, copper resistance proteins CopA, CopG and CueR were decreased in the nqr deletion strain. As a consequence, the mutant exhibited diminished resistance to copper when compared to the reference strain, as confirmed in growth studies using either glucose or mixed amino acids as carbon sources. We propose that the observed adaptations of the nqr deletion strain represent a coordinated response which counteracts a drop in transmembrane voltage that challenges V. cholerae in its different habitats. The dataset also includes raw data and MaxQuant analyses of the treatment of the V. cholerae reference strain and the nqr deletion strain with different catecholamines (WT_B - WT_D and nqr_B - nqr_D). These data will be published in a forthcoming paper.
HostingRepository	PRIDE
AnnounceDate	2018-06-21
AnnouncementXML	Submission_2018-06-21_08:38:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Pfannstiel
SpeciesList	scientific name: Vibrio cholerae O395; NCBI TaxID: 345073;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-04-03 08:14:34	ID requested
⏵ 1	2018-06-21 08:38:14	announced

Toulouse C, Metesch K, Pfannstiel J, Steuber J, Metabolic Reprogramming of Vibrio cholerae Impaired in Respiratory NADH Oxidation Is Accompanied by Increased Copper Sensitivity. J Bacteriol, 200(15):(2018) [pubmed]

curator keyword: Biological

submitter keyword: Vibrio cholerae, Na+- translocating NADH:quinone oxidoreductase, NQR, Sodium Bioenergetics, Copper Tolerance, Comparative Proteomics, LC-MS

Julia Steuber
contact affiliation	University of Hohenheim, Institute of Microbiology, 70599 Stuttgart, Germany
contact email	julia.steuber@uni-hohenheim.de
lab head
Jens Pfannstiel
contact affiliation	University of Hohenheim
contact email	Jens.Pfannstiel@uni-hohenheim.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009379
     1. Label: PRIDE project
     2. Name: Metabolic reprogramming of Vibrio cholerae impaired in respiratory NADH oxidation is accompanied with increased copper sensitivity