PXD009281 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | H4K20me2 SILAC Nucleosome Affinity Purification |
Description | Goal of this project was the identification of chromatin interacting proteins whose binding is differentially regulated by di-methylation of lysine 20 on histone H4 (H4K20me2). To achieve this unodified and H4K20me2-modified histone H4 were generated by native chemical ligation and assembled into recombinant di-nucleosomes. The di-nucleosomes were immobilized on streptavidin-coated beads via the biotinylated di-nucleosomal DNA and used for nucleosome affinity purifications to identify proteins regulated by H4K20me2 from SILAC-labelled HeLaS3 nuclear extracts (Arg10 and Lys8). SILAC affinity purifications were carried out in "forward" (heavy extract on modified nucleosome and light extract on unmodified nucleosome) and "reverse" (light extract on modified nucleosome and heavy extract on unmodified nucleosome) label-swap experiments and protein abundances were quantified by MaxQuant. |
HostingRepository | PRIDE |
AnnounceDate | 2019-02-28 |
AnnouncementXML | Submission_2019-02-28_03:25:27.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Saulius Lukauskas |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-03-21 06:24:28 | ID requested | |
⏵ 1 | 2019-02-28 03:25:29 | announced | |
Publication List
Nakamura K, Saredi G, Becker JR, Foster BM, Nguyen NV, Beyer TE, Cesa LC, Faull PA, Lukauskas S, Frimurer T, Chapman JR, Bartke T, Groth A, H4K20me0 recognition by BRCA1-BARD1 directs homologous recombination to sister chromatids. Nat Cell Biol, 21(3):311-318(2019) [pubmed] |
Keyword List
curator keyword: Technical, Biological |
submitter keyword: H4K20me2, H4K20me0, SILAC, Nucleosome Affinity Purification, SNAP |
Contact List
Till Bartke |
contact affiliation | Institute of Functional Epigenetics, Helmholtz Zentrum München, Germany |
contact email | till.bartke@helmholtz-muenchen.de |
lab head | |
Saulius Lukauskas |
contact affiliation | Helmholtz Zentrum Munich |
contact email | saulius.lukauskas@helmholtz-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD009281 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009281
- Label: PRIDE project
- Name: H4K20me2 SILAC Nucleosome Affinity Purification