PXD009281

PXD009281 is an original dataset announced via ProteomeXchange.

Title	H4K20me2 SILAC Nucleosome Affinity Purification
Description	Goal of this project was the identification of chromatin interacting proteins whose binding is differentially regulated by di-methylation of lysine 20 on histone H4 (H4K20me2). To achieve this unodified and H4K20me2-modified histone H4 were generated by native chemical ligation and assembled into recombinant di-nucleosomes. The di-nucleosomes were immobilized on streptavidin-coated beads via the biotinylated di-nucleosomal DNA and used for nucleosome affinity purifications to identify proteins regulated by H4K20me2 from SILAC-labelled HeLaS3 nuclear extracts (Arg10 and Lys8). SILAC affinity purifications were carried out in "forward" (heavy extract on modified nucleosome and light extract on unmodified nucleosome) and "reverse" (light extract on modified nucleosome and heavy extract on unmodified nucleosome) label-swap experiments and protein abundances were quantified by MaxQuant.
HostingRepository	PRIDE
AnnounceDate	2019-02-28
AnnouncementXML	Submission_2019-02-28_03:25:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Saulius Lukauskas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-21 06:24:28	ID requested
⏵ 1	2019-02-28 03:25:29	announced

Nakamura K, Saredi G, Becker JR, Foster BM, Nguyen NV, Beyer TE, Cesa LC, Faull PA, Lukauskas S, Frimurer T, Chapman JR, Bartke T, Groth A, H4K20me0 recognition by BRCA1-BARD1 directs homologous recombination to sister chromatids. Nat Cell Biol, 21(3):311-318(2019) [pubmed]

curator keyword: Technical, Biological

submitter keyword: H4K20me2, H4K20me0, SILAC, Nucleosome Affinity Purification, SNAP

Till Bartke
contact affiliation	Institute of Functional Epigenetics, Helmholtz Zentrum München, Germany
contact email	till.bartke@helmholtz-muenchen.de
lab head
Saulius Lukauskas
contact affiliation	Helmholtz Zentrum Munich
contact email	saulius.lukauskas@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD009281

PRIDE project URI

• PRIDE
  1. PXD009281
     1. Label: PRIDE project
     2. Name: H4K20me2 SILAC Nucleosome Affinity Purification