PXD009171-1
PXD009171 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | K562 Elutriation cell cycle phase fractions 2D-MS-CETSA |
Description | K562 cells were synchronized by cell size difference based Elutriation scheme into G1, S and G2 fractions. Three biological replicates were collected on different days, and assembled into a 2D-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC,54ºC and 57ºC), (Figure 1A, Methods section of the associated paper), with each set of samples heated at one temperature included in the same isobaric TMT10 set and measured at the same time on Mass Spectrometer. The labeling order of samples in the TMT10 channels is as follows: 37C/47C samples: Biorep1_G1(126), Biorep1_S(127N), Biorep1_G2(127C), Biorep2_G1(128N), Biorep2_S(128C), Biorep2_G2(129N), Biorep3_S(129C), Biorep3_G1(130N), Biorep3_G2(130C), mixed_control(131); 50C/52C/54C/57C samples: Biorep1_G1(126), Biorep1_S(127N), Biorep1_G2(127C), Biorep2_G1(128N), Biorep2_S(128C), Biorep2_G2(129N), Biorep3_G1(129C), Biorep3_S(130N), Biorep3_G2(130C), mixed_control(131); |
HostingRepository | jPOST |
AnnounceDate | 2018-05-08 |
AnnouncementXML | Submission_2018-05-07_08:00:05.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Radoslaw Sobota |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; (2S,3R)-3-hydroxyaspartic acid; iodoacetamide derivatized residue; N6-acetyl-L-lysine; deamidated L-asparagine; deamidated L-glutamine |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-03-11 21:41:25 | ID requested | |
⏵ 1 | 2018-05-07 08:00:06 | announced | |
2 | 2018-09-19 00:55:28 | announced | Updated CV, FTP location and Modification list. |
3 | 2021-02-04 17:07:35 | announced | 2021-02-04: Updated FTP location. |
4 | 2022-09-18 04:02:28 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dai L, Zhao T, Bisteau X, Sun W, Prabhu N, Lim YT, Sobota RM, Kaldis P, Nordlund P, Modulation of Protein-Interaction States through the Cell Cycle. Cell, 173(6):1481-1494.e13(2018) [pubmed] |
Keyword List
submitter keyword: MS-CETSA, cell cycle, Protein interaction state, Elutriation |
Contact List
Pär Nordlund | |
---|---|
lab head | |
Radoslaw Sobota | |
contact affiliation | IMCB A-STAR Singapore |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |