PXD009038 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sequential enrichment of phosphorylation on reversibly oxidized proteoforms – part 3 |
Description | Post-translational modifications (PTMs) are necessary for the regulation of critical processes including metabolism, signaling, and overall homeostasis. While proteins PTMs have been largely investigated independently in bottom-up proteomics methodologies, examination into how different PTMs interact, or crosstalk, will reveal a more complete understanding of the reciprocity of signaling cascades across numerous pathways. Combinatorial reversible thiol oxidation and phosphorylation in eukaryotes is largely recognized, but rigorous approaches for experimental verification are underdeveloped and must be advanced to begin meaningful definition of crosstalk in targeted pathway and systems biology research. Herein, we applied protein-level enrichment of reversibly oxidized proteoforms in Chlamydomonas reinhardtii with subsequent phosphopeptide analysis to determine the extent of phosphorylation in the redox thiol proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2018-04-23 |
AnnouncementXML | Submission_2018-04-23_05:17:58.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD009038 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Evan McConnell |
SpeciesList | scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; |
ModificationList | Deamidated; Oxidation; Acetyl; Carbamidomethyl |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-02-26 06:25:10 | ID requested | |
⏵ 1 | 2018-04-23 05:18:00 | announced | |
Publication List
McConnell EW, Werth EG, Hicks LM, The phosphorylated redox proteome of Chlamydomonas reinhardtii: Revealing novel means for regulation of protein structure and function. Redox Biol, 17():35-46(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Chlamydomonas reinhardtii, cross-talk, phosphorylation, redox proteomics, redox regulation |
Contact List
Leslie M. Hicks |
contact affiliation | Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA |
contact email | lmhicks@unc.edu |
lab head | |
Evan McConnell |
contact affiliation | Graduate Research Assistant |
contact email | evanmcc@live.unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009038
- Label: PRIDE project
- Name: Sequential enrichment of phosphorylation on reversibly oxidized proteoforms – part 3