PXD008875-1

PXD008875 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics Identifies Dual-Site Phosphorylation in an Extended Basophilic Motif Regulating FILIP1-mediated Degradation of filamin-C FILIP1 CT pull-down
Description	FILIP1 was identified as potential new filamin C interaction partner by a SILAC-based BioID experiment. Interaction of the two protein via the carboxy-terminal end of FILIP and domain 18-21 of filamin C was prooven by yeast-2-hybrid sreens and dot-blot assays, respectively. To verify this interaction by mass spectrometry, recombinantly expressed FILIP1 carboxy-term was fused via its His6-tag to Ni2+-NTA beads. Subsequently. beads were incubated with cell extracts from human skeletal muscle cells. Bands observed in SDS-PAGE from the pull-down assay were cut and analysed by LC-MS. As a result Filamin C could be identified as FILIP1 binding partner.
HostingRepository	PRIDE
AnnounceDate	2020-06-30
AnnouncementXML	Submission_2020-06-30_01:57:29.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008875
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Friedel Drepper
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Phospho; Oxidation
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-06 09:12:40	ID requested
⏵ 1	2020-06-30 01:57:30	announced

Reimann L, Schw, ä, ble AN, Fricke AL, M, ü, hlh, ä, user WWD, Leber Y, Lohanadan K, Puchinger MG, Sch, ä, uble S, Faessler E, Wiese H, Reichenbach C, Knapp B, Peikert CD, Drepper F, Hahn U, Kreutz C, van der Ven PFM, Radziwill G, Djinovi, ć, -Carugo K, F, ü, rst DO, Warscheid B, Phosphoproteomics identifies dual-site phosphorylation in an extended basophilic motif regulating FILIP1-mediated degradation of filamin-C. Commun Biol, 3(1):253(2020) [pubmed]

curator keyword: Biological

submitter keyword: FILIP1, filamin C, C2C12 myotubes

Bettina Warscheid
contact affiliation	Department of Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology and BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg im Breisgau, Germany
contact email	bettina.warscheid@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliation	AG Warscheid Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg Germany
contact email	friedel.drepper@biologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD008875

PRIDE project URI

• PRIDE
  1. PXD008875
     1. Label: PRIDE project
     2. Name: Phosphoproteomics Identifies Dual-Site Phosphorylation in an Extended Basophilic Motif Regulating FILIP1-mediated Degradation of filamin-C FILIP1 CT pull-down