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PXD008875-1

PXD008875 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomics Identifies Dual-Site Phosphorylation in an Extended Basophilic Motif Regulating FILIP1-mediated Degradation of filamin-C FILIP1 CT pull-down
DescriptionFILIP1 was identified as potential new filamin C interaction partner by a SILAC-based BioID experiment. Interaction of the two protein via the carboxy-terminal end of FILIP and domain 18-21 of filamin C was prooven by yeast-2-hybrid sreens and dot-blot assays, respectively. To verify this interaction by mass spectrometry, recombinantly expressed FILIP1 carboxy-term was fused via its His6-tag to Ni2+-NTA beads. Subsequently. beads were incubated with cell extracts from human skeletal muscle cells. Bands observed in SDS-PAGE from the pull-down assay were cut and analysed by LC-MS. As a result Filamin C could be identified as FILIP1 binding partner.
HostingRepositoryPRIDE
AnnounceDate2020-06-30
AnnouncementXMLSubmission_2020-06-30_01:57:29.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD008875
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterFriedel Drepper
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListPhospho; Oxidation
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-02-06 09:12:40ID requested
12020-06-30 01:57:30announced
Publication List
Reimann L, Schwäble AN, Fricke AL, Mühlhäuser WWD, Leber Y, Lohanadan K, Puchinger MG, Schäuble S, Faessler E, Wiese H, Reichenbach C, Knapp B, Peikert CD, Drepper F, Hahn U, Kreutz C, van der Ven PFM, Radziwill G, Djinović-Carugo K, Fürst DO, Warscheid B, Phosphoproteomics identifies dual-site phosphorylation in an extended basophilic motif regulating FILIP1-mediated degradation of filamin-C. Commun Biol, 3(1):253(2020) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: FILIP1, filamin C, C2C12 myotubes
Contact List
Bettina Warscheid
contact affiliationDepartment of Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology and BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg im Breisgau, Germany
contact emailbettina.warscheid@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliationAG Warscheid Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg Germany
contact emailfriedel.drepper@biologie.uni-freiburg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
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