PXD008797

PXD008797 is an original dataset announced via ProteomeXchange.

Title	Exoproteome analysis of Staphylococcus aureus clinical isolates belonging to different clonal complexes (C22, CC8 and CC398)
Description	Studies on S. aureus sub-populations revealed that genomes are well conserved between isolates from the same lineages despite geographic, temporal and selective diversity. However, variation of hundreds of genes can occur between isolates from different lineages and these genes could be involved in interaction with host components. In this study, we aimed to investigate the diversity of secreted virulence factors in human and zoonotic S. aureus isolates from different clonal complexes. We focused on the S. aureus clonal complexes (CC) 8 and CC22 as dominant human lineages, and CC398 as dominant livestock-associated MRSA (LA-MRSA) which is disseminating rapidly. To study the diversity of secreted virulence factors, we compared their extracellular proteomes using label-free LC-MS/MS analysis. A common protein database was created based on DNA sequencing data and PAN genome IDs.
HostingRepository	PRIDE
AnnounceDate	2018-09-11
AnnouncementXML	Submission_2018-09-17_00:15:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tobias Busche
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-30 05:54:57	ID requested
1	2018-09-11 02:27:48	announced
⏵ 2	2018-09-17 00:15:12	announced	Updated publication reference for PubMed record(s): 30201737.

Busche T, Hillion M, Van Loi V, Berg D, Walther B, Semmler T, Strommenger B, Witte W, Cuny C, Mellmann A, Holmes MA, Kalinowski J, Adrian L, Bernhardt J, Antelmann H, Isolates CC8, CC22, and CC398. Mol Cell Proteomics, 17(12):2412-2433(2018) [pubmed]

curator keyword: Biological

submitter keyword: Staphylococcus aureus, clonal complex, virulence factors, exoproteome, secretome

Haike Antelmann
contact affiliation	Freie Universität Berlin Institut für Biologie-Mikrobiologie Königin-Luise-Straße 12-16 14195 Berlin Germany ( lab head )
contact email	haike.antelmann@fu-berlin.de
lab head
Tobias Busche
contact affiliation	Freie Universität Berlin, Institute for Biology-Microbiology, D-14195 Berlin, Germany
contact email	tbusche@cebitec.uni-bielefeld.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD008797

PRIDE project URI

• PRIDE
  1. PXD008797
     1. Label: PRIDE project
     2. Name: Exoproteome analysis of Staphylococcus aureus clinical isolates belonging to different clonal complexes (C22, CC8 and CC398)