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PXD008795

PXD008795 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePeptidomics of Human Hypothalamic neurons
DescriptionThe study of peptide secretion, and identifying its control requires the use of ELISAs for measuring their levels pre and post stimulation. In place of ELISA's an LC-MS methdology was developed first to identify the correct peptides generated by the neurons, which could then be used to measure the peptide products by targetted LC-MS analysis. The Extraction was also performed on human hypothalamic tissue obtained from a tissue bank. Samples were analysed by nano LC-MS, and searched using Peaks 8.5.
HostingRepositoryPRIDE
AnnounceDate2018-08-22
AnnouncementXMLSubmission_2018-08-22_08:38:24.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD008795
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRichard Kay
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListAmidated; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-30 05:50:50ID requested
12018-08-22 08:38:25announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Hypothalamic neurons, POMC, Peptidomics
Contact List
Florian Merkle
contact affiliationUniversity of Cambridge
contact emailfm436@medschl.cam.ac.uk
lab head
Richard Kay
contact affiliationUniversity of Cambridge
contact emailrgk27@medschl.cam.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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