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PXD008653-1

PXD008653 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDocosahexaenoic and Arachidonic Free Fatty Acids Exert Different Antitumor Activities on Colorectal Cancer Cells
DescriptionTwo PUFA, docosahexaenoic (DHA, 22: 6n3) and arachidonic acids (ARA, 20: 4n6), as well as their derivatives such as eicosanoids, regulate different activities, which include changes in receptor signaling, the composition of rafts, cell metabolism, and membrane structures. These also modify the function of transcription factors and their target genes, a key step essential for the function of FA-activated signaling. This work is focused to determine the antitumor actions of these compounds linked to the regulation of gene transcription on HT-29 colorectal cancer. For this, we performed antiproliferative antitumour assay, LDH breakage test, caspase-3 production, and proteome changes were assessed by SWATH-MS quantitative proteomics followed by pathway analysis in order to find out which molecular mechanisms were being affected. In all cases tested, DHA exercised antitumor actions to a higher extent than ARA, acting mainly by means of down-regulating most of the proteasome system particles, while ARA presented a heavy effect on all the six DNA replication helicase particles but did not affect proteasome. The results indicated that both DHA and ARA stopped colorectal cancer growth and proliferation, thus they represent the ideal candidates as chemopreventive agents.
HostingRepositoryPRIDE
AnnounceDate2022-01-28
AnnouncementXMLSubmission_2022-01-28_05:13:28.183.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIgnacio Ortea
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-11 02:27:44ID requested
12022-01-28 05:13:28announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biomedical
submitter keyword: colorectal cancer
proteomics
PUFA
DHA
ARA
SWATH
Contact List
Ignacio Ortea
contact affiliationProteomics Unit, IMIBIC
contact emailignacio.ortea@imibic.org
lab head
Ignacio Ortea
contact affiliationIMIBIC
contact emailiortea@gmail.com
dataset submitter
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Dataset FTP location
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