PXD008361 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Preservation methods and PBS washing affect the AML proteome |
Description | In the current study, we have studied the effect of three storage and preparation protocols on two AML-derived cell lines: 1) cryopreservation of viable cells for storage in liquid nitrogen, 2) cell pellet storage in liquid nitrogen and 3) freshly SDS lysed cell lysate stored at -°80. We further studied the effect cell pellet wash with cold PBS had on the AML patient cell lysate, aiming to remove remaining contaminants from FBS and blood. |
HostingRepository | PRIDE |
AnnounceDate | 2018-01-19 |
AnnouncementXML | Submission_2018-01-19_03:59:48.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Elise Aasebo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-12-04 06:53:54 | ID requested | |
⏵ 1 | 2018-01-19 03:59:49 | announced | |
2 | 2019-02-26 08:15:57 | announced | Updated publication reference for PubMed record(s): 29351208. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Technical |
submitter keyword: AML, PBS, DMSO, storage conditions |
Contact List
Frode Selheim |
contact affiliation | Proteomics Unit, Department of Biomedicine, University of Bergen, Norway |
contact email | frode.selheim@uib.no |
lab head | |
Elise Aasebo |
contact affiliation | PROBE |
contact email | elise.aasebo@uib.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008361
- Label: PRIDE project
- Name: Preservation methods and PBS washing affect the AML proteome