PXD008361

PXD008361 is an original dataset announced via ProteomeXchange.

Title	Preservation methods and PBS washing affect the AML proteome
Description	In the current study, we have studied the effect of three storage and preparation protocols on two AML-derived cell lines: 1) cryopreservation of viable cells for storage in liquid nitrogen, 2) cell pellet storage in liquid nitrogen and 3) freshly SDS lysed cell lysate stored at -°80. We further studied the effect cell pellet wash with cold PBS had on the AML patient cell lysate, aiming to remove remaining contaminants from FBS and blood.
HostingRepository	PRIDE
AnnounceDate	2018-01-19
AnnouncementXML	Submission_2019-02-26_08:15:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elise Aasebo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-04 06:53:54	ID requested
1	2018-01-19 03:59:49	announced
⏵ 2	2019-02-26 08:15:57	announced	Updated publication reference for PubMed record(s): 29351208.

Wangen R, Aaseb, ø E, Trentani A, D, ø, skeland SO, Bruserud Ø, Selheim F, Hernandez-Valladares M, Preservation Method and Phosphate Buffered Saline Washing Affect the Acute Myeloid Leukemia Proteome. Int J Mol Sci, 19(1):(2018) [pubmed]

curator keyword: Technical

submitter keyword: AML, PBS, DMSO, storage conditions

Frode Selheim
contact affiliation	Proteomics Unit, Department of Biomedicine, University of Bergen, Norway
contact email	frode.selheim@uib.no
lab head
Elise Aasebo
contact affiliation	PROBE
contact email	elise.aasebo@uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD008361

PRIDE project URI

• PRIDE
  1. PXD008361
     1. Label: PRIDE project
     2. Name: Preservation methods and PBS washing affect the AML proteome