PXD008298-1

PXD008298 is an original dataset announced via ProteomeXchange.

Title	Dynamic Yin-Yang regulation network of O-GlcNAcylation and phosphorylation mediatesvernalization response for flowering in winter wheat, part 2
Description	Protein post-translational modification (PTM) increases the functional diversity of the proteome and regulates numerous biological processes in eukaryotes. Two types of PTMs, O-linked-acetyl glucosamine modification (O-GlcNAc) and phosphorylation have been identified on the same amino acid, are considered as Yin-Yang modification for their antagonistic function recently. Vernalization, a prolonged cold exposure promoted flowering, is important for grain yield in temperate cereals, such as winter wheat. O-GlcNAcylation on TaGRP2 and phosphorylation on VER2 are involved in regulation of vernalization response (VRN) genes. However, less is known about how plant senses vernalization with general Yin-Yang modifications. Here we report that altering O-GlcNAc signaling by chemical inhibitors could change the vernalization response and affect flowering transition. Furthermore, we enriched O-GlcNAcylated and phosphorylated peptides from winter wheat plumules at different processing time points during vernalization by Lectin weak affinity chromatography (LWAC) and iTRAQ-TiO2, respectively. In total, about 200 O-GlcNAcylated proteins and 124 differential expressed phosphorylated proteins were identified by Mass Spectrum (MS). Based on GO enrichment, the identified O-GlcNAcylated proteins are mainly involved in response to abiotic stimulus and hormone, metabolic processing and gene expression. While dynamic phosphorylated proteins during vernalization participate in translation, transcription and metabolic processing. Of note, 31 proteins with both phosphorylation and O-GlcNAcylation modification were identified. Among them, TaGRP2 was further confirmed to participate in regulation of vernalization promoted flowering. The global modification profiles and genetic data at specific regulator suggested that the dynamic network of O-GlcNAcylation and phosphorylation on the key nodes regulate vernalization response and mediate flowering in wheat.
HostingRepository	PRIDE
AnnounceDate	2021-05-18
AnnouncementXML	Submission_2021-05-17_21:53:56.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shujuan Xu
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	carbamoylated residue; phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-27 01:44:29	ID requested
⏵ 1	2021-05-17 21:53:58	announced

Xu S, Xiao J, Yin F, Guo X, Xing L, Xu Y, Chong K, -GlcNAcylation and Phosphorylation Mediate Vernalization Response for Flowering in Winter Wheat. Plant Physiol, 180(3):1436-1449(2019) [pubmed]

curator keyword: Biological

submitter keyword: O-GlcNAcylation modification, phosphorylation modification, proteomics

Kang Chong
contact affiliation	Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences
contact email	chongk@ibcas.ac.cn
lab head
Shujuan Xu
contact affiliation	Institute of Botany, CAS
contact email	xushujuan11@mails.ucas.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008298
     1. Label: PRIDE project
     2. Name: Dynamic Yin-Yang regulation network of O-GlcNAcylation and phosphorylation mediatesvernalization response for flowering in winter wheat, part 2