PXD008163 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Depleted Prefractionated Human Plasma for Data Independent Acquisition Spectral Library |
Description | We established a spectral library for data-independent analysis of human plasma samples, using a pooled plasma sample from Danish adults. The samples were prepared for proteome analysis using an in-house developed protocol consuming one microliter of plasma per sample, and the tryptic digest and subsequent C18 purification was performed in 96-well plates. The spectral library consists of plasma which was analyzed neat (i.e. non-treated), and top 14 depleted. The samples were subjected to high pH reversed-phase peptide fractionated using C18 tips (8 fraction) and a HPLC system (20 fraction). The resulting peptides were analyzed on a Q Exactive with a capflow reversed-phase C18 LC-MS/MS setup in data-dependent mode. The final spectral library contains 21788 precursors and 1000 proteins (both at FDR<0.01). |
HostingRepository | PRIDE |
AnnounceDate | 2018-04-20 |
AnnouncementXML | Submission_2018-04-20_01:47:03.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tue Bjerg Bennike |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-11-08 01:25:38 | ID requested | |
⏵ 1 | 2018-04-20 01:47:04 | announced | |
Publication List
Bennike TB, Bellin MD, Xuan Y, Stensballe A, M, ø, ller FT, Beilman GJ, Levy O, Cruz-Monserrate Z, Andersen V, Steen J, Conwell DL, Steen H, A Cost-Effective High-Throughput Plasma and Serum Proteomics Workflow Enables Mapping of the Molecular Impact of Total Pancreatectomy with Islet Autotransplantation. J Proteome Res, 17(5):1983-1992(2018) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: human, plasma, spectral library, depletion, prefractionated, high pH, LC-MSMS |
Contact List
Hanno Steen |
contact affiliation | Departments of Pathology, Boston Children’s Hospital and Harvard Medical School, Boston, MA, USA |
contact email | hanno.steen@childrens.harvard.edu |
lab head | |
Tue Bjerg Bennike |
contact affiliation | Aalborg University |
contact email | tbe@hst.aau.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008163 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008163
- Label: PRIDE project
- Name: Depleted Prefractionated Human Plasma for Data Independent Acquisition Spectral Library