PXD008089

PXD008089 is an original dataset announced via ProteomeXchange.

Title	Metaproteomics and direct Protein-SIF of the deep-sea mussel Bathymodiolus childressi and its bacterial symbionts
Description	Bathymodiolus childressi is a species of deep-sea mussels found predominantly in the Gulf of Mexico. It colonizes cold seeps such as brine pool and oil seeps. The success of these animals in such environment is thought to be due to the symbiotic association of the mussel host with several species of bacteria. The aim of this study is to understand the role of the different partners involved in the symbiotic system using various “-omics” approaches. In addition to protein identification we used the mass spectrometry data generated and submitted with this project to derive the stable carbon isotope ratios for the different members of the symbiosis using the direct Protein-SIF method. The respective isotope pattern file and SIF computation files are included with this submission.
HostingRepository	PRIDE
AnnounceDate	2019-09-25
AnnouncementXML	Submission_2020-02-21_02:37:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tjorven Hinzke
SpeciesList	scientific name: Archaea; NCBI TaxID: 2157; scientific name: Viruses; NCBI TaxID: 10239; scientific name: Bathymodiolus childressi; NCBI TaxID: 114452; scientific name: Bacteria; NCBI TaxID: 2;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-31 07:04:14	ID requested
1	2019-09-25 07:36:53	announced
2	2020-01-27 00:33:00	announced	2020-01-27: Updated publication reference for PubMed record(s): 31562384.
⏵ 3	2020-02-21 02:37:08	announced	2020-01-27: Updated publication reference for PubMed record(s): 31562384. 2020-02-21: Updated publication reference for PubMed record(s): 31562384.

Assi, é A, Leisch N, Meier DV, Gruber-Vodicka H, Tegetmeyer HE, Meyerdierks A, Kleiner M, Hinzke T, Joye S, Saxton M, Dubilier N, Petersen JM, Horizontal acquisition of a patchwork Calvin cycle by symbiotic and free-living Campylobacterota (formerly Epsilonproteobacteria). ISME J, 14(1):104-122(2020) [pubmed]

curator keyword: Biological

submitter keyword: Chemosynthetic symbiosis, bacteria-animal symbiosis, cold seeps, epsilonproteobacteria, stable isotope fingerprinting, Calvin cycle, stable carbon isotopes, methanotrophic symbionts, 2D-LC-MS/MS, 1D-LC-MS/MS, Q Exactive Plus

Manuel Kleiner
contact affiliation	Department of Geoscience, University of Calgary, Calgary, AB, Canada; Department of Plant and Microbial Biology, North Carolina State University, Raleigh, North Carolina, USA
contact email	manuel_kleiner@ncsu.edu
lab head
Tjorven Hinzke
contact affiliation	University of Greifswald, Greifswald Intitute of Marine Biotechnology
contact email	tjorven.hinzke@stud.uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD008089

PRIDE project URI

• PRIDE
  1. PXD008089
     1. Label: PRIDE project
     2. Name: Metaproteomics and direct Protein-SIF of the deep-sea mussel Bathymodiolus childressi and its bacterial symbionts