PXD007988-1

PXD007988 is an original dataset announced via ProteomeXchange.

Title	T-cell synaptic ectosomes relay signals through microcluster transfer
Description	Extracellular vesicles (EV) are proposed to transfer information between cells. In the immunological synapse T cell receptor (TCR) interaction with peptide-MHC drives microcluster formation and signaling that is terminated in part through sorting of TCR into EVs that bud into the synapse, synaptic ectosomes (SE). SE isolated on bead supported lipid bilayers (BSLB) contained TCR and a number of EV/retrovirus markers including CD317, a retroviral restriction factor. CD278 and CD154 were enriched in SE by respective ligands, expanding potential functions. Mass Spec profiling of SE identified endosomal sorting complexes required for transport (ESCRT) machinery and TCR microcluster components. Stochastic reconstruction microscopy (STORM) revealed TCR colocalization with CD278 and CD317, but segregation from CD154 within single SE. SE activated dendritic cells (DC) and CD154 recapitulated this effect only when presented on liposomes at SE density.
HostingRepository	PRIDE
AnnounceDate	2019-09-12
AnnouncementXML	Submission_2019-09-12_01:39:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roman Fischer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-18 02:09:04	ID requested
⏵ 1	2019-09-12 01:55:15	announced
2	2019-10-02 00:59:01	announced	2019-10-02: Updated publication reference for PubMed record(s): 31469364.

Saliba DG, C, é, spedes-Donoso PF, B, á, lint Š, Compeer EB, Korobchevskaya K, Valvo S, Mayya V, Kvalvaag A, Peng Y, Dong T, Tognoli ML, O'Neill E, Bonham S, Fischer R, Kessler BM, Dustin ML, Composition and structure of synaptic ectosomes exporting antigen receptor linked to functional CD40 ligand from helper T cells. Elife, 8():(2019) [pubmed]

curator keyword: Biological

submitter keyword: Exosomes

Roman Fischer
contact affiliation	Target Discovery Institute, University of Oxford
contact email	roman.fischer@ndm.ox.ac.uk
lab head
Roman Fischer
contact affiliation	University of Oxford
contact email	roman.fischer@ndm.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD007988

PRIDE project URI

• PRIDE
  1. PXD007988
     1. Label: PRIDE project
     2. Name: T-cell synaptic ectosomes relay signals through microcluster transfer