PXD007957-3
PXD007957 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | TOTAL AND DIFFERENTIAL PROTEOME OF AXILLARY BUDS OF SUGARCANE CULTIVAR RB867515 IN DIFFERENT CUTS |
Description | The objective of this work was to know the effect of sugarcane cultivation on protein expression during the sugarcane cutting cycles, characterizing the total and differential proteome of axillary buds of the plant cane and fourth ratoon of the cultivar RB867515. The total proteins were extracted in triplicate by the modified TCA/acetone method for use in axillary sugarcane buds. Protein samples were submitted to denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and stained with comassie blue. Each lane of the polyacrylamide gel was fractionated (11 slice) and treated for purification and isolation of the polypeptides. Tryptic digestion was performed on each fragment with subsequent UPLC separation (Nano Acquity). The polypeptides were analyzed on Waters® Micromass® ESI-Q-Tof micro ™ mass spectrometer. Protein samples from the axillary buds at different cut stages showed distinct bands patterns in the SDS-PAGE gel. Mass spectrometry data were analyzed by MASCOT tool (http://www.matrixscience.com). |
HostingRepository | jPOST |
AnnounceDate | 2019-08-04 |
AnnouncementXML | Submission_2022-09-18_03:33:15.896.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rone Charles Maranho |
SpeciesList | scientific name: Saccharum hybrid cultivar RB867515; NCBI TaxID: 1890409; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
Instrument | Q-Tof micro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-10-12 08:22:08 | ID requested | |
1 | 2019-08-04 07:32:59 | announced | |
2 | 2021-02-04 17:12:09 | announced | 2021-02-04: Updated FTP location. |
⏵ 3 | 2022-09-18 03:33:16 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Saccharum spp, Longevity of sugarcane, Meristem proteome |
Contact List
Rone Charles Maranho | |
---|---|
lab head | |
Rone Charles Maranho | |
contact affiliation | State University of Maringá |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000331/ |