PXD007957

PXD007957 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	TOTAL AND DIFFERENTIAL PROTEOME OF AXILLARY BUDS OF SUGARCANE CULTIVAR RB867515 IN DIFFERENT CUTS
Description	The objective of this work was to know the effect of sugarcane cultivation on protein expression during the sugarcane cutting cycles, characterizing the total and differential proteome of axillary buds of the plant cane and fourth ratoon of the cultivar RB867515. The total proteins were extracted in triplicate by the modified TCA/acetone method for use in axillary sugarcane buds. Protein samples were submitted to denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and stained with comassie blue. Each lane of the polyacrylamide gel was fractionated (11 slice) and treated for purification and isolation of the polypeptides. Tryptic digestion was performed on each fragment with subsequent UPLC separation (Nano Acquity). The polypeptides were analyzed on Waters® Micromass® ESI-Q-Tof micro ™ mass spectrometer. Protein samples from the axillary buds at different cut stages showed distinct bands patterns in the SDS-PAGE gel. Mass spectrometry data were analyzed by MASCOT tool (http://www.matrixscience.com).
HostingRepository	jPOST
AnnounceDate	2019-08-04
AnnouncementXML	Submission_2022-09-18_03:33:15.896.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rone Charles Maranho
SpeciesList	scientific name: Saccharum hybrid cultivar RB867515; NCBI TaxID: 1890409;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q-Tof micro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-12 08:22:08	ID requested
1	2019-08-04 07:32:59	announced
2	2021-02-04 17:12:09	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 03:33:16	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Saccharum spp, Longevity of sugarcane, Meristem proteome

submitter keyword: Saccharum spp, Longevity of sugarcane, Meristem proteome

Contact List

Rone Charles Maranho
lab head
Rone Charles Maranho
contact affiliation	State University of Maringá
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000331/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000331/