PXD007873-2
PXD007873 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | TDP-43 in ALS/FTLD studied by a novel aggregate extraction method |
Description | Accumulation of abnormally phosphorylated TDP-43 (pTDP-43) is the main pathological finding characterizing affected neurons in most patients with amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). At least four different subtypes of FTLD-TDP have been described, based on the morphology and neuroanatomical distribution of pathological TDP-43 accumulations. To understand the molecular basis of this heterogeneity that correlates with clinical presentations, we developed SarkoSpin, a new method for the biochemical isolation of pathological TDP-43 from complex tissues. Using postmortem samples of 79 patients and controls, we show that SarkoSpin allows the physical separation of pTDP-43 from ~99.8% of total proteins, including the extreme bulk of physiological TDP-43. Pathological TDP-43 extracted from different disease subtypes forms large and buoyant assemblies of distinct densities and 3-dimentional shapes that correlate with specific neuropathological classifications. |
HostingRepository | PRIDE |
AnnounceDate | 2019-12-13 |
AnnouncementXML | Submission_2019-12-13_07:31:58.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paul Boersema |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-09-29 05:42:15 | ID requested | |
1 | 2018-10-10 04:07:38 | announced | |
⏵ 2 | 2019-12-13 07:32:00 | announced | 2019-12-13: Updated publication reference for PubMed record(s): 30559480. |
Publication List
Laferri, è, re F, Maniecka Z, P, é, rez-Berlanga M, Hruska-Plochan M, Gilhespy L, Hock EM, Wagner U, Afroz T, Boersema PJ, Barmettler G, Foti SC, Asi YT, Isaacs AM, Al-Amoudi A, Lewis A, Stahlberg H, Ravits J, De Giorgi F, Ichas F, Bezard E, Picotti P, Lashley T, Polymenidou M, TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates. Nat Neurosci, 22(1):65-77(2019) [pubmed] |
Keyword List
ProteomeXchange project tag: Protein Misfolding and Aggregation (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
curator keyword: Biological, Biomedical |
submitter keyword: TDP-43, ALS, FTLD |
Contact List
Paola Picotti | |
---|---|
contact affiliation | ETH Zurich |
contact email | paola.picotti@bc.biol.ethz.ch |
lab head | |
Paul Boersema | |
contact affiliation | ETH Zurich, Inst. of Biochemistry |
contact email | paul.boersema@bc.biol.ethz.ch |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007873 |
PRIDE project URI |
Repository Record List
[ + ]