PXD007702-1
PXD007702 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Affinity purification to find Arabidopsis TSN1 and TSN2 interacting proteins |
Description | As the first step towards a better understanding of the regulation and molecular function of SGs and PBs in plants, we performed affinity purification and mass spectrometry of proteins constituting these mRNP complexes in Arabidopsis. Since TSN has been ascribed a scaffolding role in recruiting other proteins to the stress-induced cytoplasmic mRNP foci, we used two Arabidopsis TSN homologs, TSN1 and TSN2, as baits to purify plant mRNP complex-associated proteins. Our experiments revealed that direct homologs of key components of yeast and animal SGs and PBs are bound to TSN in plants. Interestingly, we also found a group of TSN-interacting proteins that appear to be novel or plant-specific components of mRNP complexes. |
HostingRepository | jPOST |
AnnounceDate | 2023-02-25 |
AnnouncementXML | Submission_2023-02-24_07:00:04.775.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Emilio Gutierrez Beltran |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | unknown modification; S-carboxymethyl-L-cysteine |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-09-08 06:47:25 | ID requested | |
⏵ 1 | 2023-02-24 07:00:05 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mRNP complexes, stress granules, processing bodies |
Contact List
Peter V. Bozhkov and Emilio Gutierrez-Beltran | |
---|---|
lab head | |
Emilio Gutierrez Beltran | |
contact affiliation | University of Sevilla |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000316/ |