PXD007702

PXD007702 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Affinity purification to find Arabidopsis TSN1 and TSN2 interacting proteins
Description	As the first step towards a better understanding of the regulation and molecular function of SGs and PBs in plants, we performed affinity purification and mass spectrometry of proteins constituting these mRNP complexes in Arabidopsis. Since TSN has been ascribed a scaffolding role in recruiting other proteins to the stress-induced cytoplasmic mRNP foci, we used two Arabidopsis TSN homologs, TSN1 and TSN2, as baits to purify plant mRNP complex-associated proteins. Our experiments revealed that direct homologs of key components of yeast and animal SGs and PBs are bound to TSN in plants. Interestingly, we also found a group of TSN-interacting proteins that appear to be novel or plant-specific components of mRNP complexes.
HostingRepository	jPOST
AnnounceDate	2023-02-25
AnnouncementXML	Submission_2023-02-24_07:00:04.775.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilio Gutierrez Beltran
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	unknown modification; S-carboxymethyl-L-cysteine
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-09-08 06:47:25	ID requested
⏵ 1	2023-02-24 07:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: mRNP complexes, stress granules, processing bodies

submitter keyword: mRNP complexes, stress granules, processing bodies

Contact List

Peter V. Bozhkov and Emilio Gutierrez-Beltran
lab head
Emilio Gutierrez Beltran
contact affiliation	University of Sevilla
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000316/