<<< Full experiment listing

PXD007141-1

PXD007141 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRefinement of synapses without postsynaptic activity: Role for a 4E-BP dependent mechanism
DescriptionRefinement of synaptic connections during development requires both the elimination of some inputs and the strengthening of others. It is well accepted that this competitive process takes place throughout the nervous system and relies on synaptic activity. However, the mechanisms that link activity to refinement remain largely unresolved. Here we demonstrate that refinement of preganglionic axons in sympathetic ganglia, a process that depends unequivocally on synaptic activity, involves eukaryotic initiation factor 4E binding protein (4E-BP), a protein that regulates cap-dependent translation. In mice lacking the 3 nicotinic receptor subunit, silent synapses between preganglionic axons and postsynaptic nicotinic neurons do not refine unless synaptic activity is restored, or surprisingly, when 4E-BP is absent. Our findings demonstrate that synapses can refine in the absence of synaptic activity during development and identify 4E-BP as a critical player in this process.
HostingRepositoryMassIVE
AnnounceDate2018-02-09
AnnouncementXMLSubmission_2018-02-09_17:34:38.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBirgit Schilling
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListunknown modification: NA
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-07-26 19:51:35ID requested
12018-02-09 17:34:38announced
Publication List
no publication
Keyword List
submitter keyword: postsynaptic density, protein expression, data-independent acquisition
Contact List
Birgit Schilling
contact affiliationBuck Institute
contact emailbschilling@buckinstitute.org
lab head
Birgit Schilling
contact affiliationBuck Institute
contact emailbschilling@buckinstitute.org
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081386