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PXD007127-1

PXD007127 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular alterations elicited by chronic exposure to cigarette smoke and chewing tobacco in oral keratinocytes
DescriptionTobacco is mainly consumed in two different forms (smoking and chewing) that vary in their composition and methods of intake. Despite being the leading cause of oral cancer, the molecular mechanisms resulting in malignancy upon tobacco exposure are yet to be fully elucidated. We therefore sought to compare the molecular alterations in oral keratinocytes exposed to smoke and chewing tobacco. OKF6/TERT1 cells were exposed to cigarette smoke condensate or chewing tobacco for progressively increasing durations (2, 4, 6 and 8 months). We employed a TMT-based quantitative proteomics approach to investigate the adverse effects of chronic cigarette smoke or chewing tobacco exposure in oral keratinocytes. LC/MS3 analysis resulted in the quantification of 5,342 proteins and 2,821 proteins in cigarette smoke and chewing tobacco exposed cells, respectively. Upstream regulator analysis indicates the involvement of distinct regulators in CSC exposed cells compared to STE exposed cells. In addition, exome sequencing revealed discrete genetic alterations in cells exposed to each insult. Current analysis defines a clear distinction in the molecular dysregulation in oral cells in response to different tobacco-based insults. Some of the proteins dysregulated in cigarette smoke or chewing tobacco exposed cells may serve as potential early detection biomarkers which could aid in stratification of patients based on tobacco usage history.
HostingRepositoryPRIDE
AnnounceDate2019-11-08
AnnouncementXMLSubmission_2019-11-08_09:15:12.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAditi Chatterjee
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-07-25 07:51:38ID requested
12019-11-08 09:15:14announced
Publication List
Rajagopalan P, Patel K, Jain AP, Nanjappa V, Datta KK, Subbannayya T, Mangalaparthi KK, Kumari A, Manoharan M, Coral K, Murugan S, Nair B, Prasad TSK, Mathur PP, Gupta R, Gupta R, Khanna-Gupta A, Califano J, Sidransky D, Gowda H, Chatterjee A, Molecular alterations associated with chronic exposure to cigarette smoke and chewing tobacco in normal oral keratinocytes. Cancer Biol Ther, 19(9):773-785(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Orbitrap Fusion, high-throughput, carcinogenesis, smoking, chronic exposure
Contact List
Aditi Chatterjee
contact affiliationInstitute of Bioinformatics, Unit 1, 7th floor, Discoverer building, ITPL, Whitefield, Bangalore - 560066, Karnataka, India
contact emailaditixchatterjee@gmail.com
lab head
Aditi Chatterjee
contact affiliationInstitute of Bioinformatics, Bangalore, Karnataka, India
contact emailaditixchatterjee@gmail.com
dataset submitter
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PRIDE project URI
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