PXD007062 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome analyses define marked differences between N. caninum isolates throughout the tachyzoite lytic cycle |
Description | Neospora caninum has been recognised as the main cause of transmissible abortion in cattle worldwide. Although the pathogenesis of this disease remains largely unknown, different factors associated to both the host and the parasite have influence in the dynamics of the disease. Intraspecific variation in virulence have been widely shown among N. caninum isolates. However, the molecular basis that govern such variability and the outcome of the infection have not been well-established yet. This study aims to improve the knowledge on the factors governing intra-specific variation in virulence in N. caninum. Quantitative label free LC-MS/MS was used to investigate proteome differences between the high virulent isolate Nc-Spain7 and the low virulent isolate Nc-Spain1H through tachyzoite lytic cycle: at the end of invasion (12 hours), during exponential growth (36 hours) and at early egress (56 hours). The results showed the higher differences in abundance of proteins at invasion and egress, 77 and 62 proteins, respectively. At 36 h, during active parasite replication only 19 proteins were found differentially abundant between isolates. Microneme protein repertoire, involved in parasite invasion and egress, was more abundant in Nc-Spain1H isolate, which displays a lower invasion rate. Soluble effectors related to Toxoplasma gondii virulence (ROP and GRA), proteins of carbohydrate and fatty acid metabolism and stress responses also showed different abundance between isolates. Differential expression analysis by RNAseq technology during tachyzoite egress from the host-cell (56 hours) was also performed showing an expression profile of genes associated with the bradyzoite stage the low virulent and low growing NcSpain1H isolate. The differences in proteome and RNA expression profiles between these two isolates reveal interesting insights into likely mechanisms involved in specific phenotypic traits and virulence in N. caninum. |
HostingRepository | PRIDE |
AnnounceDate | 2017-11-17 |
AnnouncementXML | Submission_2017-11-17_03:59:31.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dong Xia |
SpeciesList | scientific name: Neospora caninum (Coccidian parasite); NCBI TaxID: 29176; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-07-17 05:32:45 | ID requested | |
⏵ 1 | 2017-11-17 03:59:32 | announced | |
2 | 2017-12-11 04:18:49 | announced | Updated publication reference for PubMed record(s): 29154927. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Neospora, Tachyzoite, Lytic cycle, Virulence. |
Contact List
Jonathan Wastling |
contact affiliation | Faculty of Natural Sciences, Keele University. |
contact email | j.wastling@keele.ac.uk |
lab head | |
Dong Xia |
contact affiliation | Infection Biology |
contact email | d.xia@liv.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD007062
- Label: PRIDE project
- Name: Proteome analyses define marked differences between N. caninum isolates throughout the tachyzoite lytic cycle