PXD007062

PXD007062 is an original dataset announced via ProteomeXchange.

Title	Proteome analyses define marked differences between N. caninum isolates throughout the tachyzoite lytic cycle
Description	Neospora caninum has been recognised as the main cause of transmissible abortion in cattle worldwide. Although the pathogenesis of this disease remains largely unknown, different factors associated to both the host and the parasite have influence in the dynamics of the disease. Intraspecific variation in virulence have been widely shown among N. caninum isolates. However, the molecular basis that govern such variability and the outcome of the infection have not been well-established yet. This study aims to improve the knowledge on the factors governing intra-specific variation in virulence in N. caninum. Quantitative label free LC-MS/MS was used to investigate proteome differences between the high virulent isolate Nc-Spain7 and the low virulent isolate Nc-Spain1H through tachyzoite lytic cycle: at the end of invasion (12 hours), during exponential growth (36 hours) and at early egress (56 hours). The results showed the higher differences in abundance of proteins at invasion and egress, 77 and 62 proteins, respectively. At 36 h, during active parasite replication only 19 proteins were found differentially abundant between isolates. Microneme protein repertoire, involved in parasite invasion and egress, was more abundant in Nc-Spain1H isolate, which displays a lower invasion rate. Soluble effectors related to Toxoplasma gondii virulence (ROP and GRA), proteins of carbohydrate and fatty acid metabolism and stress responses also showed different abundance between isolates. Differential expression analysis by RNAseq technology during tachyzoite egress from the host-cell (56 hours) was also performed showing an expression profile of genes associated with the bradyzoite stage the low virulent and low growing NcSpain1H isolate. The differences in proteome and RNA expression profiles between these two isolates reveal interesting insights into likely mechanisms involved in specific phenotypic traits and virulence in N. caninum.
HostingRepository	PRIDE
AnnounceDate	2017-11-17
AnnouncementXML	Submission_2017-12-11_04:18:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dong Xia
SpeciesList	scientific name: Neospora caninum (Coccidian parasite); NCBI TaxID: 29176;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-17 05:32:45	ID requested
1	2017-11-17 03:59:32	announced
⏵ 2	2017-12-11 04:18:49	announced	Updated publication reference for PubMed record(s): 29154927.

Horcajo P, Xia D, Randle N, Collantes-Fern, á, ndez E, Wastling J, Ortega-Mora LM, Regidor-Cerrillo J, Integrative transcriptome and proteome analyses define marked differences between Neospora caninum isolates throughout the tachyzoite lytic cycle. J Proteomics, 180():108-119(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Neospora, Tachyzoite, Lytic cycle, Virulence.

Jonathan Wastling
contact affiliation	Faculty of Natural Sciences, Keele University.
contact email	j.wastling@keele.ac.uk
lab head
Dong Xia
contact affiliation	Infection Biology
contact email	d.xia@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007062
     1. Label: PRIDE project
     2. Name: Proteome analyses define marked differences between N. caninum isolates throughout the tachyzoite lytic cycle