PXD007055-1

PXD007055 is an original dataset announced via ProteomeXchange.

Title	Chromokinesin KIF4A teams up with STMN1 to regulate abscission in a SUMO-dependent manner
Description	Cell division ends when two daughter cells physically separate via abscission, the cleavage of the intercellular bridge. It is currently less clear how the anti-parallel microtubule bundles bridging daughter cells are severed. Here, we provide evidence for a novel abscission mechanism. We found that the chromokinesin KIF4A accumulates adjacent to the midbody during cytokinesis and is required for efficient abscission. KIF4A interacts with the microtubule destabilizer STMN1 as identified by mass spectrometry, providing mechanistic insight. This interaction is enhanced by SUMOylation of KIF4A. We mapped lysine 460 in KIF4A as SUMO acceptor site and employed CRISPR-Cas9 mediated genome editing to block SUMO conjugation of endogenous KIF4A, resulting in a delay in cytokinesis. Combined, our work provides novel insight in abscission regulation by a KIF4A, STMN1 and SUMO triad.
HostingRepository	PRIDE
AnnounceDate	2020-07-14
AnnouncementXML	Submission_2020-07-13_22:48:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sabine Cuijpers
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-17 01:28:44	ID requested
⏵ 1	2020-07-13 22:48:50	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: KIF4A, SUMO, STMN1, mitosis, cytokinesis, abscission, human, LC-MS/MS

Alfred C. O. Vertegaal
contact affiliation	Department of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, the Netherlands
contact email	vertegaal@lumc.nl
lab head
Sabine Cuijpers
contact affiliation	Leiden University Medical Center
contact email	s.a.g.cuijpers@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD007055

PRIDE project URI

• PRIDE
  1. PXD007055
     1. Label: PRIDE project
     2. Name: Chromokinesin KIF4A teams up with STMN1 to regulate abscission in a SUMO-dependent manner