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PXD007055 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChromokinesin KIF4A teams up with STMN1 to regulate abscission in a SUMO-dependent manner
DescriptionCell division ends when two daughter cells physically separate via abscission, the cleavage of the intercellular bridge. It is currently less clear how the anti-parallel microtubule bundles bridging daughter cells are severed. Here, we provide evidence for a novel abscission mechanism. We found that the chromokinesin KIF4A accumulates adjacent to the midbody during cytokinesis and is required for efficient abscission. KIF4A interacts with the microtubule destabilizer STMN1 as identified by mass spectrometry, providing mechanistic insight. This interaction is enhanced by SUMOylation of KIF4A. We mapped lysine 460 in KIF4A as SUMO acceptor site and employed CRISPR-Cas9 mediated genome editing to block SUMO conjugation of endogenous KIF4A, resulting in a delay in cytokinesis. Combined, our work provides novel insight in abscission regulation by a KIF4A, STMN1 and SUMO triad.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSabine Cuijpers
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-07-17 01:28:44ID requested
12020-07-13 22:48:50announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: KIF4A, SUMO, STMN1, mitosis, cytokinesis, abscission, human, LC-MS/MS
Contact List
Alfred C. O. Vertegaal
contact affiliationDepartment of Molecular Cell Biology, Leiden University Medical Center, 2300 RC Leiden, the Netherlands
contact emailvertegaal@lumc.nl
lab head
Sabine Cuijpers
contact affiliationLeiden University Medical Center
contact emails.a.g.cuijpers@lumc.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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