PXD006993 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics after spinal cord injury in a regenerative and a non-regenerative stage in Xenopus laevis |
Description | The capacity to regenerate the spinal cord after an injury is a coveted trait that only a limited group of non-mammalian organisms can achieve. In Xenopus laevis, this capacity is only present during larval or tadpole stages, but is absent during postmetamorphic frog stages. This provides an excellent model for comparative studies between a regenerative and a non-regenerative stage to identify the cellular and molecular mechanisms that explain the difference in regenerative potential. Here, we used iTRAQ chemistry to obtain a quantitative proteome of the spinal cord 1 day after a transection injury, and used sham operated animals as controls. We quantified a total of 6,384 proteins, with 172 showing significant differential expression in the regenerative stage and 240 in the non-regenerative stage, with an overlap of only 14 proteins. Functional enrichment analysis revealed that while the regenerative stage downregulated synapse/vesicle and mitochondrial proteins, the non-regenerative stage upregulated lipid metabolism proteins, and downregulated ribosomal and translation control proteins. Furthermore, STRING network analysis showed that proteins belonging to these groups are highly interconnected, providing interesting candidates for future functional studies. |
HostingRepository | PRIDE |
AnnounceDate | 2018-01-25 |
AnnouncementXML | Submission_2018-01-25_02:39:14.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Liangliang Sun |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-07-12 01:53:59 | ID requested | |
⏵ 1 | 2018-01-25 02:39:15 | announced | |
Publication List
Lee-Liu D, Sun L, Dovichi NJ, Larra, í, n J, . Mol Cell Proteomics, 17(4):592-606(2018) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: spinal cord injury, regenerative and a non-regenerative stage, Xenopus laevis, quantitative proteomics |
Contact List
Liangliang Sun |
contact affiliation | Michigan State University |
contact email | lsun@chemistry.msu.edu |
lab head | |
Liangliang Sun |
contact affiliation | Michigan State University |
contact email | lsun@chemistry.msu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006993
- Label: PRIDE project
- Name: Quantitative proteomics after spinal cord injury in a regenerative and a non-regenerative stage in Xenopus laevis