PXD006993

PXD006993 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics after spinal cord injury in a regenerative and a non-regenerative stage in Xenopus laevis
Description	The capacity to regenerate the spinal cord after an injury is a coveted trait that only a limited group of non-mammalian organisms can achieve. In Xenopus laevis, this capacity is only present during larval or tadpole stages, but is absent during postmetamorphic frog stages. This provides an excellent model for comparative studies between a regenerative and a non-regenerative stage to identify the cellular and molecular mechanisms that explain the difference in regenerative potential. Here, we used iTRAQ chemistry to obtain a quantitative proteome of the spinal cord 1 day after a transection injury, and used sham operated animals as controls. We quantified a total of 6,384 proteins, with 172 showing significant differential expression in the regenerative stage and 240 in the non-regenerative stage, with an overlap of only 14 proteins. Functional enrichment analysis revealed that while the regenerative stage downregulated synapse/vesicle and mitochondrial proteins, the non-regenerative stage upregulated lipid metabolism proteins, and downregulated ribosomal and translation control proteins. Furthermore, STRING network analysis showed that proteins belonging to these groups are highly interconnected, providing interesting candidates for future functional studies.
HostingRepository	PRIDE
AnnounceDate	2018-01-25
AnnouncementXML	Submission_2018-01-25_02:39:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liangliang Sun
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-12 01:53:59	ID requested
⏵ 1	2018-01-25 02:39:15	announced

Lee-Liu D, Sun L, Dovichi NJ, Larra, í, n J, . Mol Cell Proteomics, 17(4):592-606(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: spinal cord injury, regenerative and a non-regenerative stage, Xenopus laevis, quantitative proteomics

Liangliang Sun
contact affiliation	Michigan State University
contact email	lsun@chemistry.msu.edu
lab head
Liangliang Sun
contact affiliation	Michigan State University
contact email	lsun@chemistry.msu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006993
     1. Label: PRIDE project
     2. Name: Quantitative proteomics after spinal cord injury in a regenerative and a non-regenerative stage in Xenopus laevis