PXD006856-1

PXD006856 is an original dataset announced via ProteomeXchange.

Title	Naa80 acetylates the actin N-terminus impacting the actin cytoskeleton and cell motility
Description	Eukaryotic actins undergo several post-translational modifications during their maturation to enable a fully functional cytoskeletal network. A unique N-terminal processing is conserved in the animal kingdom and includes the removal of the first one or two amino acids as well as N-terminal acetylation of the newly exposed acidic N-termini. These events have been known for decades, but the mechanism and function of actin N-terminal acetylation are not well understood due to the lack of information on the catalysing N-terminal acetyltransferase (NAT). Here, we show that NAA80, previously known as NAT6 or FUS-2,is a unique, animal kingdom-specific NAT that exclusively N-terminally acetylates actins. In vitro, the lack of actin N-terminal acetylation impedes the actin polymerization rate due to decreased formin-mediated filament elongation, while the depolymerisation rate is unaffected. Human NAA80 knockout cells display a complete loss of actin N-terminal acetylation and a reorganization of the actin cytoskeleton. We reveal Naa80 as the enzyme that specifically N-terminally acetylates actin the most abundant protein in animal cells, and that this modification controls cellular dynamics and cytoskeletal functions. In this project acetylation of actins was investigated with the use of LC-MS/MS and MS1 quantitation of the Total Ion Current signal.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_11:35:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evy Timmerman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-03 03:35:30	ID requested
⏵ 1	2018-10-24 11:35:56	announced

Drazic A, Aksnes H, Marie M, Boczkowska M, Varland S, Timmerman E, Foyn H, Glomnes N, Rebowski G, Impens F, Gevaert K, Dominguez R, Arnesen T, NAA80 is actin's N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility. Proc Natl Acad Sci U S A, 115(17):4399-4404(2018) [pubmed]

curator keyword: Biological

submitter keyword: human, NAT, actin, N-terminal acetylation

Thomas Arnesen
contact affiliation	Department of Molecular Biology, University of Bergen, N-5020 Bergen
contact email	Thomas.Arnesen@uib.no
lab head
Evy Timmerman
contact affiliation	Ghent University - VIB
contact email	evy.timmerman@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006856
     1. Label: PRIDE project
     2. Name: Naa80 acetylates the actin N-terminus impacting the actin cytoskeleton and cell motility