PXD006831 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomics of DIA1-overexpressing HEK cells and protemics of probable DIA1 interactors |
Description | Many kinases are still “orphans”, i.e. knowledge about their substrates, and often also about the processes they regulate, is lacking. Here, DIA1/C3orf58, a member of a novel predicted kinase-like family is subjected to phosphoproteomics analysis in order to cast light on its signalling pathways. LC MS/MS proteomics approach with Ti phosphopeptide enrichment is applied to membrane fractions of DIA1-overexpressing and control HEK cells, and phosphosites dependent on DIA1 presence are elucidated. Most of The these phosphosites belonged to CK2- and proline-directed kinase types. In parallel, proteomics of proteins immunoprecipitated with DIA1 provided its probable interactors. Intersection of the DIA1-affected phosphoproteins and interactors provides putative DIA1 substrates, including calnexin . This pilot study data, mapped on literature, provides basis for deeper studies of DIA1 signalling. |
HostingRepository | PRIDE |
AnnounceDate | 2018-03-26 |
AnnouncementXML | Submission_2018-03-26_04:57:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michał Kistowski |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; monohydroxylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-06-29 03:29:26 | ID requested | |
⏵ 1 | 2018-03-26 04:57:51 | announced | |
2 | 2018-04-23 02:37:05 | announced | Updated publication reference for PubMed record(s): 29666759. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: Novel kinases, phosphoproteomics, mass spectrometry, secretory pathway |
Contact List
Michał Dadlez |
contact affiliation | Institute of Biochemistry and Biophysics PAS |
contact email | michald@ibb.waw.pl |
lab head | |
Michał Kistowski |
contact affiliation | Institute of Biochemistry and Biophysics PAS |
contact email | mkisto@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006831
- Label: PRIDE project
- Name: Phosphoproteomics of DIA1-overexpressing HEK cells and protemics of probable DIA1 interactors