PXD006831

PXD006831 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of DIA1-overexpressing HEK cells and protemics of probable DIA1 interactors
Description	Many kinases are still “orphans”, i.e. knowledge about their substrates, and often also about the processes they regulate, is lacking. Here, DIA1/C3orf58, a member of a novel predicted kinase-like family is subjected to phosphoproteomics analysis in order to cast light on its signalling pathways. LC MS/MS proteomics approach with Ti phosphopeptide enrichment is applied to membrane fractions of DIA1-overexpressing and control HEK cells, and phosphosites dependent on DIA1 presence are elucidated. Most of The these phosphosites belonged to CK2- and proline-directed kinase types. In parallel, proteomics of proteins immunoprecipitated with DIA1 provided its probable interactors. Intersection of the DIA1-affected phosphoproteins and interactors provides putative DIA1 substrates, including calnexin . This pilot study data, mapped on literature, provides basis for deeper studies of DIA1 signalling.
HostingRepository	PRIDE
AnnounceDate	2018-03-26
AnnouncementXML	Submission_2018-04-23_02:37:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michał Kistowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-29 03:29:26	ID requested
1	2018-03-26 04:57:51	announced
⏵ 2	2018-04-23 02:37:05	announced	Updated publication reference for PubMed record(s): 29666759.

Hareza A, Bakun M, Ś, widerska B, Dudkiewicz M, Koscielny A, Bajur A, Jaworski J, Dadlez M, Paw, ł, owski K, Phosphoproteomic insights into processes influenced by the kinase-like protein DIA1/C3orf58. PeerJ, 6():e4599(2018) [pubmed]

curator keyword: Biological

submitter keyword: Novel kinases, phosphoproteomics, mass spectrometry, secretory pathway

Michał Dadlez
contact affiliation	Institute of Biochemistry and Biophysics PAS
contact email	michald@ibb.waw.pl
lab head
Michał Kistowski
contact affiliation	Institute of Biochemistry and Biophysics PAS
contact email	mkisto@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD006831

PRIDE project URI

• PRIDE
  1. PXD006831
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of DIA1-overexpressing HEK cells and protemics of probable DIA1 interactors