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PXD006831 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomics of DIA1-overexpressing HEK cells and protemics of probable DIA1 interactors
DescriptionMany kinases are still “orphans”, i.e. knowledge about their substrates, and often also about the processes they regulate, is lacking. Here, DIA1/C3orf58, a member of a novel predicted kinase-like family is subjected to phosphoproteomics analysis in order to cast light on its signalling pathways. LC MS/MS proteomics approach with Ti phosphopeptide enrichment is applied to membrane fractions of DIA1-overexpressing and control HEK cells, and phosphosites dependent on DIA1 presence are elucidated. Most of The these phosphosites belonged to CK2- and proline-directed kinase types. In parallel, proteomics of proteins immunoprecipitated with DIA1 provided its probable interactors. Intersection of the DIA1-affected phosphoproteins and interactors provides putative DIA1 substrates, including calnexin . This pilot study data, mapped on literature, provides basis for deeper studies of DIA1 signalling.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichał Kistowski
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-06-29 03:29:26ID requested
12018-03-26 04:57:51announced
22018-04-23 02:37:05announcedUpdated publication reference for PubMed record(s): 29666759.
Publication List
Hareza A, Bakun M, Świderska B, Dudkiewicz M, Koscielny A, Bajur A, Jaworski J, Dadlez M, Pawłowski K, Phosphoproteomic insights into processes influenced by the kinase-like protein DIA1/C3orf58. PeerJ, 6():e4599(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Novel kinases, phosphoproteomics, mass spectrometry, secretory pathway
Contact List
Michał Dadlez
contact affiliationInstitute of Biochemistry and Biophysics PAS
contact emailmichald@ibb.waw.pl
lab head
Michał Kistowski
contact affiliationInstitute of Biochemistry and Biophysics PAS
contact emailmkisto@gmail.com
dataset submitter
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