PXD006790 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma |
Description | FAM46C is one of the most frequently mutated genes in multiple myeloma (MM). Here, using a combination of in vitro and in vivo approaches, we demonstrate that FAM46C encodes an active non-canonical poly(A) polymerase which enhances mRNA stability and gene expression. Reintroduction of active FAM46C into MM cell lines, but not its catalytically-inactive mutant, leads to broad polyadenylation and stabilization of mRNAs strongly enriched with those encoding endoplasmic reticulum-targeted proteins and induces cell death. Moreover, silencing of FAM46C in MM cells expressing WT protein enhance cell proliferation. Finally, using a FAM46C-FLAG knock-in mouse strain we show that the FAM46C protein is strongly induced during activation of primary splenocytes and that B lymphocytes isolated from newly generated FAM46C KO mice proliferate faster than those isolated from their WT littermates. Concluding, our data clearly indicate that FAM46C works as an onco-suppressor, with the specificity for B-lymphocyte lineage from which multiple myeloma originates. |
HostingRepository | PRIDE |
AnnounceDate | 2018-03-09 |
AnnouncementXML | Submission_2018-03-09_06:28:31.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dominik Cysewski |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-06-23 02:08:04 | ID requested | |
⏵ 1 | 2018-03-09 06:28:32 | announced | |
Publication List
Mroczek S, Chlebowska J, Kuli, ń, ski TM, Gewartowska O, Gruchota J, Cysewski D, Liudkovska V, Borsuk E, Nowis D, Dziembowski A, The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma. Nat Commun, 8(1):619(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: FAM46C, multiple myeloma, polyadenylation, anemia, B lymphocytes, mouse, LC-MSMS, |
Contact List
Andrzej Dziembowski |
contact affiliation | Laboraotry of RNA Biology and Functional Genomics, Insititue of Biochemistry and Biophysics Polish Academy od Sciences, Warsaw, Poland |
contact email | andrzej.dziembowski.ibb@gmail.com |
lab head | |
Dominik Cysewski |
contact affiliation | Institute of Biochemistry and Biophysics Polish Academy of Sciences |
contact email | dominikcysewski@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD006790
- Label: PRIDE project
- Name: The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma