PXD006790

PXD006790 is an original dataset announced via ProteomeXchange.

Title	The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma
Description	FAM46C is one of the most frequently mutated genes in multiple myeloma (MM). Here, using a combination of in vitro and in vivo approaches, we demonstrate that FAM46C encodes an active non-canonical poly(A) polymerase which enhances mRNA stability and gene expression. Reintroduction of active FAM46C into MM cell lines, but not its catalytically-inactive mutant, leads to broad polyadenylation and stabilization of mRNAs strongly enriched with those encoding endoplasmic reticulum-targeted proteins and induces cell death. Moreover, silencing of FAM46C in MM cells expressing WT protein enhance cell proliferation. Finally, using a FAM46C-FLAG knock-in mouse strain we show that the FAM46C protein is strongly induced during activation of primary splenocytes and that B lymphocytes isolated from newly generated FAM46C KO mice proliferate faster than those isolated from their WT littermates. Concluding, our data clearly indicate that FAM46C works as an onco-suppressor, with the specificity for B-lymphocyte lineage from which multiple myeloma originates.
HostingRepository	PRIDE
AnnounceDate	2018-03-09
AnnouncementXML	Submission_2018-03-09_06:28:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominik Cysewski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-23 02:08:04	ID requested
⏵ 1	2018-03-09 06:28:32	announced

Mroczek S, Chlebowska J, Kuli, ń, ski TM, Gewartowska O, Gruchota J, Cysewski D, Liudkovska V, Borsuk E, Nowis D, Dziembowski A, The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma. Nat Commun, 8(1):619(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: FAM46C, multiple myeloma, polyadenylation, anemia, B lymphocytes, mouse, LC-MSMS,

Andrzej Dziembowski
contact affiliation	Laboraotry of RNA Biology and Functional Genomics, Insititue of Biochemistry and Biophysics Polish Academy od Sciences, Warsaw, Poland
contact email	andrzej.dziembowski.ibb@gmail.com
lab head
Dominik Cysewski
contact affiliation	Institute of Biochemistry and Biophysics Polish Academy of Sciences
contact email	dominikcysewski@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD006790

PRIDE project URI

• PRIDE
  1. PXD006790
     1. Label: PRIDE project
     2. Name: The non-canonical poly(A) polymerase FAM46C acts as an onco-suppressor in multiple myeloma