PXD006711

PXD006711 is an original dataset announced via ProteomeXchange.

Title	SNAIL is a key regulator of rhabdomyosarcoma progression and myogenic differentiation
Description	Rhabdomyosarcoma (RMS) is a frequent non-epithelial tumor of soft tissue that originates from a myogenic differentiation defect. Expression of SNAIL transcription factor is elevated in the alveolar subtype of RMS, characterized by a low myogenic differentiation status and high aggressiveness. SNAIL affects RMS metastasis by reorganization of actin cytoskeleton, regulation of ezrin expression and chemotaxis to HGF and SDF-1. The differentiation of human RMS diminishes SNAIL level. SNAIL silencing completely abolishes the growth of human RMS xenotransplants. SNAIL inhibits myogenic differentiation of RMS by binding to the MYF5 promoter, suppressing its expression, displacing MYOD from canonical to alternative E-box sequences and regulating myomiRs expression. SNAIL silencing allows the re-expression of MYF5 and canonical MYOD binding, promoting RMS cell myogenic differentiation. These novel results open potential avenues for the development of innovative therapeutic strategies based on SNAIL silencing.
HostingRepository	PRIDE
AnnounceDate	2020-07-14
AnnouncementXML	Submission_2020-07-18_18:36:53.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006711
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Urszula Jankowska
SpeciesList	scientific name: Homo sapiens (human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	micrOTOF-Q II

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-14 03:00:17	ID requested
1	2020-07-13 22:49:43	announced
⏵ 2	2020-07-18 18:36:54	announced	2020-07-19: Updated publication reference for PubMed record(s): 32664538.

Skrzypek K, Kot M, Konieczny P, Nieszporek A, Kusienicka A, Lasota M, Bobela W, Jankowska U, K, ę, dracka-Krok S, Majka M, SNAIL Promotes Metastatic Behavior of Rhabdomyosarcoma by Increasing EZRIN and AKT Expression and Regulating MicroRNA Networks. Cancers (Basel), 12(7):(2020) [pubmed]

submitter keyword: SNAIL (SNAI1), rhabdomyosarcoma (RMS), myogenic differentiation, MYOD, MYF5, metastasis, myomiRs

Sylwia Kędracka-Krok
contact affiliation	Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
contact email	sylwi.kedracka-krok@uj.edu.pl
lab head
Urszula Jankowska
contact affiliation	Jagiellonian University
contact email	urszula.jankowska@uj.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD006711

PRIDE project URI

• PRIDE
  1. PXD006711
     1. Label: PRIDE project
     2. Name: SNAIL is a key regulator of rhabdomyosarcoma progression and myogenic differentiation