PXD006696 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of HDA14-dependent substrates in the Arabidopsis thaliana leaf acetylome |
Description | Lysine acetylation is a common post-translational modification in eukaryotes and prokaryotes which is known to be involved in the regulation of various cellular processes, such as transcriptional activation, metabolic signaling, and energy homeostasis. Nevertheless, its role in plant primary metabolism, and photosynthesis in particular, is not well understood. Lysine acetylation is catalyzed by acetyltransferases (KATs) and removed by corresponding deacetylases (KDACs). The Arabidopsis thaliana genome encodes for at least 16 KATs and 18 KDACs, belonging to seven different gene families. The cellular functions of these enzymes have been explored only partially, describing effects of single enzymes and their interaction with specific targets. HDA14 is a KDAC expressed primarily in chloroplasts which suggests an involvement in the regulation of photosynthesis or related metabolic processes. In this dataset we aimed to identify substrates of the histone deacetylase 14 in a global manner by comparing the leaf lysine acetylome of a hda14 KO plant to a wild-type under normal growth conditions. Proteins were extracted and digested using an adapted FASP procedure, peptides were dimethyl-labeled, ZIC-HILIC fractionated and lysine-acetylated peptides were antibody-enriched. Peptide identification and quantitative data analysis was carried out using MaxQuant. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-25 |
AnnouncementXML | Submission_2017-10-25_02:05:21.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Katharina Kramer |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | 4x(2)H labeled dimethylated L-lysine; acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-06-12 00:34:35 | ID requested | |
⏵ 1 | 2017-10-25 02:05:22 | announced | |
Publication List
Hartl M, F, ü, ß, l M, Boersema PJ, Jost JO, Kramer K, Bakirbas A, Sindlinger J, Pl, ö, chinger M, Leister D, Uhrig G, Moorhead GB, Cox J, Salvucci ME, Schwarzer D, Mann M, Finkemeier I, . Mol Syst Biol, 13(10):949(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Arabidopsis, lysine acetylation, histone deacetylases, photosynthesis, RuBisCO activase |
Contact List
Prof. Iris Finkemeier |
contact affiliation | Plant Physiology Group Westfälische WIlhelms-Universität Münster Germany |
contact email | iris.finkemeier@uni-muenster.de |
lab head | |
Katharina Kramer |
contact affiliation | Max Planck Institute for Plant Breeding Research |
contact email | kramer@mpipz.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006696
- Label: PRIDE project
- Name: Identification of HDA14-dependent substrates in the Arabidopsis thaliana leaf acetylome