PXD006696

PXD006696 is an original dataset announced via ProteomeXchange.

Title	Identification of HDA14-dependent substrates in the Arabidopsis thaliana leaf acetylome
Description	Lysine acetylation is a common post-translational modification in eukaryotes and prokaryotes which is known to be involved in the regulation of various cellular processes, such as transcriptional activation, metabolic signaling, and energy homeostasis. Nevertheless, its role in plant primary metabolism, and photosynthesis in particular, is not well understood. Lysine acetylation is catalyzed by acetyltransferases (KATs) and removed by corresponding deacetylases (KDACs). The Arabidopsis thaliana genome encodes for at least 16 KATs and 18 KDACs, belonging to seven different gene families. The cellular functions of these enzymes have been explored only partially, describing effects of single enzymes and their interaction with specific targets. HDA14 is a KDAC expressed primarily in chloroplasts which suggests an involvement in the regulation of photosynthesis or related metabolic processes. In this dataset we aimed to identify substrates of the histone deacetylase 14 in a global manner by comparing the leaf lysine acetylome of a hda14 KO plant to a wild-type under normal growth conditions. Proteins were extracted and digested using an adapted FASP procedure, peptides were dimethyl-labeled, ZIC-HILIC fractionated and lysine-acetylated peptides were antibody-enriched. Peptide identification and quantitative data analysis was carried out using MaxQuant.
HostingRepository	PRIDE
AnnounceDate	2017-10-25
AnnouncementXML	Submission_2017-10-25_02:05:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katharina Kramer
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	4x(2)H labeled dimethylated L-lysine; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-12 00:34:35	ID requested
⏵ 1	2017-10-25 02:05:22	announced

Hartl M, F, ü, ß, l M, Boersema PJ, Jost JO, Kramer K, Bakirbas A, Sindlinger J, Pl, ö, chinger M, Leister D, Uhrig G, Moorhead GB, Cox J, Salvucci ME, Schwarzer D, Mann M, Finkemeier I, . Mol Syst Biol, 13(10):949(2017) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, lysine acetylation, histone deacetylases, photosynthesis, RuBisCO activase

Prof. Iris Finkemeier
contact affiliation	Plant Physiology Group Westfälische WIlhelms-Universität Münster Germany
contact email	iris.finkemeier@uni-muenster.de
lab head
Katharina Kramer
contact affiliation	Max Planck Institute for Plant Breeding Research
contact email	kramer@mpipz.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006696
     1. Label: PRIDE project
     2. Name: Identification of HDA14-dependent substrates in the Arabidopsis thaliana leaf acetylome