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PXD006680-1

PXD006680 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells
Description4-Oxo-2-nonenal (ONE) derived from lipid peroxidation modifies nucleophiles and transduces redox signaling by its reactions with proteins. However, the molecular interactions between ONE and complex proteomes and their dynamics in situ remain largely unknown. Here we de-scribe a quantitative chemoproteomic analysis of protein adduction by ONE in cells, in which the cellular target profile of ONE is mimicked by its alkynyl surrogate. The analysis reveals four types of ONE-derived modifications in cells, including ketoamide and Schiff-base adducts to lysine, Michael adducts to cysteine, and a novel pyrrole adducts to cysteine. ONE-derived adducts co-localize and crosstalk with many histone marks and redox sensitive sites. All four types of modifications derived from ONE can be reversed site-specifically in cells. In summary, our study provides much-needed mechanistic insights into the cellular signaling and potential toxicities associated with this important lipid derived electrophile.
HostingRepositoryPRIDE
AnnounceDate2022-02-24
AnnouncementXMLSubmission_2022-02-24_05:35:21.283.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJing Yang
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-06-08 03:37:40ID requested
12022-02-24 05:35:21announced
22023-11-14 08:49:24announced2023-11-14: Updated project metadata.
Publication List
10.1074/MCP.RA117.000116;
Keyword List
curator keyword: Biological
submitter keyword: Chemoproteomics, 4-Oxo-2-nonenal,Click chemistry
Contact List
Jing Yang
contact affiliationNCPSB, China
contact emailyangjing54@hotmail.com
lab head
Jing Yang
contact affiliationNational Center for Protein Sciences, Beijing
contact emailyangjing54@hotmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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