PXD006399-1
PXD006399 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | APP phosphorylation by LRRK2 |
| Description | To investigate the global phosphorylation sites of Amyloid precursor protein (APP) by Leucine-rich repeat kinase 2 (LRRK2) protein kinase activities and explore the potential substrates involving neural loss in Parkinson’s Disease (PD), we performed a phosphoproteomic analysis of APP by LC-MS/MS method. LRRK2 wide-type full length protein and recombinant Human APP751 were mixed with kinase assay buffer, followed by incubation. Laemmmli buffer was added to stop the reaction and loaded to a 4-12% SDS-PAGE gel. In-gel digestion was performed according to standard protocol. The LC-MS/MS analysis was carried out on an Orbitrap Fusion. The MS and MS/MS spectra from the raw data were sent to the Mascot database search engine against the Uniport Human protein database. The quantification of phosphorylation was based on the extracted ion chromatograph of the triply charged non-phosphorylated and phosphorylated peptide containing T724. |
| HostingRepository | jPOST |
| AnnounceDate | 2018-04-28 |
| AnnouncementXML | Submission_2018-04-27_08:00:05.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhongcan Chen |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | (2S,3R)-3-hydroxyaspartic acid; iodoacetamide derivatized residue; N6-acetyl-L-lysine |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2017-04-27 09:42:50 | ID requested | |
| ⏵ 1 | 2018-04-27 08:00:06 | announced | |
| 2 | 2018-09-19 00:11:38 | announced | Updated CV, FTP location and Modification list. |
| 3 | 2021-02-04 16:44:02 | announced | 2021-02-04: Updated FTP location. |
| 4 | 2022-09-18 03:47:06 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Human, Substrate, Phosphorylation, PD |
Contact List
| Eng King Tan; Li Zeng | |
|---|---|
| lab head | |
| Zhongcan Chen | |
| contact affiliation | National Neuroscience Institute |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
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