PXD006390 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | EGF-dependent ubiquitinome, phosphorylation and interactome analysis in control and CYLD-deficient cells |
Description | Cylindromatosis tumor suppressor protein (CYLD) is deubiquitinase, best known as an essential negative regulator of the NFkB pathway. Previous studies have suggested an involvement of CYLD in epidermal growth factor (EGF)-dependent signal transduction as well, as it was found enriched within the tyrosine-phosphorylated complexes in cells stimulated with the growth factor. EGF receptor (EGFR) signaling participates in central cellular processes and its tight regulation, partly through ubiquitination cascades, is decisive for a balanced cellular homeostasis. Here, using a combination of mass spectrometry-based quantitative proteomic approaches with biochemical and immunofluorescence strategies, we demonstrate the involvement of CYLD in the regulation of the ubiquitination events triggered by EGF. Our data show that CYLD regulates the magnitude of ubiquitination of several major effectors of the EGFR pathway by assisting the recruitment of the ubiquitin ligase Cbl-b to the activated EGFR complex. Notably, the deficiency of CYLD impairs the interaction of EGFR with Cbl-b, which leads to a reduced ubiquitination of the receptor followed by its decreased degradation. This represents a previously uncharacterized strategy exerted by this deubiquitinase and tumors pressor for the negative regulation of a tumorigenic signaling pathway. |
HostingRepository | PRIDE |
AnnounceDate | 2017-06-05 |
AnnouncementXML | Submission_2017-06-05_02:47:44.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michele Puglia |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-04-26 06:11:29 | ID requested | |
⏵ 1 | 2017-06-05 02:47:45 | announced | |
Publication List
Sanchez-Quiles V, Akimov V, Osinalde N, Francavilla C, Puglia M, Barrio-Hernandez I, Kratchmarova I, Olsen JV, Blagoev B, Cylindromatosis Tumor Suppressor Protein (CYLD) Deubiquitinase is Necessary for Proper Ubiquitination and Degradation of the Epidermal Growth Factor Receptor. Mol Cell Proteomics, 16(8):1433-1446(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: CYLD, EGFR, PRM, HeLA, Ubiquitin, phosphorylation |
Contact List
Blagoy Blagoev |
contact affiliation | Professor, PhD Department of Biochemistry and Molecular Biology Southern Denmark University |
contact email | bab@bmb.sdu.dk |
lab head | |
Michele Puglia |
contact affiliation | University of Southern Denmark |
contact email | michelep@bmb.sdu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006390
- Label: PRIDE project
- Name: EGF-dependent ubiquitinome, phosphorylation and interactome analysis in control and CYLD-deficient cells