PXD006390

PXD006390 is an original dataset announced via ProteomeXchange.

Title	EGF-dependent ubiquitinome, phosphorylation and interactome analysis in control and CYLD-deficient cells
Description	Cylindromatosis tumor suppressor protein (CYLD) is deubiquitinase, best known as an essential negative regulator of the NFkB pathway. Previous studies have suggested an involvement of CYLD in epidermal growth factor (EGF)-dependent signal transduction as well, as it was found enriched within the tyrosine-phosphorylated complexes in cells stimulated with the growth factor. EGF receptor (EGFR) signaling participates in central cellular processes and its tight regulation, partly through ubiquitination cascades, is decisive for a balanced cellular homeostasis. Here, using a combination of mass spectrometry-based quantitative proteomic approaches with biochemical and immunofluorescence strategies, we demonstrate the involvement of CYLD in the regulation of the ubiquitination events triggered by EGF. Our data show that CYLD regulates the magnitude of ubiquitination of several major effectors of the EGFR pathway by assisting the recruitment of the ubiquitin ligase Cbl-b to the activated EGFR complex. Notably, the deficiency of CYLD impairs the interaction of EGFR with Cbl-b, which leads to a reduced ubiquitination of the receptor followed by its decreased degradation. This represents a previously uncharacterized strategy exerted by this deubiquitinase and tumors pressor for the negative regulation of a tumorigenic signaling pathway.
HostingRepository	PRIDE
AnnounceDate	2017-06-05
AnnouncementXML	Submission_2017-06-05_02:47:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Puglia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-26 06:11:29	ID requested
⏵ 1	2017-06-05 02:47:45	announced

Sanchez-Quiles V, Akimov V, Osinalde N, Francavilla C, Puglia M, Barrio-Hernandez I, Kratchmarova I, Olsen JV, Blagoev B, Cylindromatosis Tumor Suppressor Protein (CYLD) Deubiquitinase is Necessary for Proper Ubiquitination and Degradation of the Epidermal Growth Factor Receptor. Mol Cell Proteomics, 16(8):1433-1446(2017) [pubmed]

curator keyword: Biological

submitter keyword: CYLD, EGFR, PRM, HeLA, Ubiquitin, phosphorylation

Blagoy Blagoev
contact affiliation	Professor, PhD Department of Biochemistry and Molecular Biology Southern Denmark University
contact email	bab@bmb.sdu.dk
lab head
Michele Puglia
contact affiliation	University of Southern Denmark
contact email	michelep@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD006390

PRIDE project URI

• PRIDE
  1. PXD006390
     1. Label: PRIDE project
     2. Name: EGF-dependent ubiquitinome, phosphorylation and interactome analysis in control and CYLD-deficient cells